ArticleApplied and environmental microbiology2005
Spatially resolved characterization of biogenic manganese oxide production within a bacterial biofilm.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 1 citations in OpenAlex.
- Structure of HEPES-Reduced δ‑MnOChemistry of materials : a publication of the American Chemical Society · 2026Article
- Enhanced Manganese Oxidation at the Biofilm-Fluid Interface Drives Pore-Scale Patterns in Mineral Precipitation.Environmental science & technology · 2026Article
- Role of biofilm during groundwater biofiltration of manganese.Scientific reports · 2025Article
- Population-level control of two manganese oxidases expands the niche for bacterial manganese biomineralization.NPJ biofilms and microbiomes · 2025Article
- Organic buffers act as reductants of abiotic and biogenic manganese oxides.Scientific reports · 2023Article
- Chemical-state distributions in charged LiCoOScientific reports · 2023Article
- Biologically Assisted One-Step Synthesis of Electrode Materials for Li-Ion Batteries.Microorganisms · 2023Article
- Diversity of Mixotrophic Neutrophilic Thiosulfate- and Iron-Oxidizing Bacteria from Deep-Sea Hydrothermal Vents.Microorganisms · 2022Article
- Microbially Induced Mineralization of Layered Mn Oxides Electroactive in Li Batteries.Frontiers in microbiology · 2020Article
- Mn oxide formation by phototrophs: Spatial and temporal patterns, with evidence of an enzymatic superoxide-mediated pathway.Scientific reports · 2019Article
- Article
- Preparation and representation of recombinant Mn-ferritin flower-like spherical aggregates from marine invertebrates.PloS one · 2015Article
- Effects of exogenous pyoverdines on Fe availability and their impacts on Mn(II) oxidation by Pseudomonas putida GB-1.Frontiers in microbiology · 2014Article
- Pyoverdine synthesis by the Mn(II)-oxidizing bacterium Pseudomonas putida GB-1.Frontiers in microbiology · 2014Article
- CotA, a multicopper oxidase from Bacillus pumilus WH4, exhibits manganese-oxidase activity.PloS one · 2013Article
- Constraints on superoxide mediated formation of manganese oxides.Frontiers in microbiology · 2013Article
- Synthesis and characterization of a novel extracellular biogenic manganese oxide (bixbyite-like Mn₂O₃) nanoparticle by isolated Acinetobacter sp.Current microbiology · 2011Article
- Sorption and distribution of copper in unsaturated Pseudomonas putida CZ1 biofilms as determined by X-ray fluorescence microscopy.Applied and environmental microbiology · 2011Article
- Biogenic origin of polymetallic nodules from the Clarion-Clipperton Zone in the Eastern Pacific Ocean: electron microscopic and EDX evidence.Marine biotechnology (New York, N.Y.)Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas putida strain MnB1, a biofilm-forming bacterial culture, was used as a model for the study of bacterial Mn oxidation in freshwater and soil environments. The oxidation of aqueous Mn+2 [Mn+2(aq)] by P. putida was characterized by spatially and temporally resolving the oxidation state of Mn in the presence of a bacterial biofilm, using scanning transmission X-ray microscopy (STXM) combined with near-edge X-ray absorption fine structure (NEXAFS) spectroscopy at the Mn L2,3 absorption edges. Subsamples were collected from growth flasks containing 0.1 and 1 mM total Mn at 16, 24, 36, and 48 h after inoculation. Immediately after collection, the unprocessed hydrated subsamples were imaged at a 40-nm resolution. Manganese NEXAFS spectra were extracted from X-ray energy sequences of STXM images (stacks) and fit with linear combinations of well-characterized reference spectra to obtain quantitative relative abundances of Mn(II), Mn(III), and Mn(IV). Careful consideration was given to uncertainty in the normalization of the reference spectra, choice of reference compounds, and chemical changes due to radiation damage. The STXM results confirm that Mn+2(aq) was removed from solution by P. putida and was concentrated as Mn(III) and Mn(IV) immediately adjacent to the bacterial cells. The Mn precipitates were completely enveloped by bacterial biofilm material. The distribution of Mn oxidation states was spatially heterogeneous within and between the clusters of bacterial cells. Scanning transmission X-ray microscopy is a promising tool for advancing the study of hydrated interfaces between minerals and bacteria, particularly in cases where the structure of bacterial biofilms needs to be maintained.
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