ArticleApplied and environmental microbiology2005
Heterologous production, assembly, and secretion of a minicellulosome by Clostridium acetobutylicum ATCC 824.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 70 citations in OpenAlex.
- Improvement of arabinoxylan degradation in Clostridium saccharobutylicum DSM 13864Applied microbiology and biotechnology · 2025Article
- Combinatorial optimization of the hybrid cellulase complex structure designed from modular libraries.Scientific reports · 2024Article
- Current progress on engineering microbial strains and consortia for production of cellulosic butanol through consolidated bioprocessing.Microbial biotechnology · 2023Review
- Editorial: Microorganisms for Consolidated 2nd Generation Biorefining.Frontiers in microbiology · 2022Article
- Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization.Microbial biotechnology · 2020Review
- Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824.Biotechnology for biofuels · 2016Article
- Genome and Transcriptome of Clostridium phytofermentans, Catalyst for the Direct Conversion of Plant Feedstocks to Fuels.PloS one · 2015Article
- Artificial symbiosis for acetone-butanol-ethanol (ABE) fermentation from alkali extracted deshelled corn cobs by co-culture of Clostridium beijerinckii and Clostridium cellulovorans.Microbial cell factories · 2014Article
- Improving activity of minicellulosomes by integration of intra- and intermolecular synergies.Biotechnology for biofuels · 2013Article
- Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 824.Biotechnology for biofuels · 2013Article
- Functional heterologous expression of an engineered full length CipA from Clostridium thermocellum in Thermoanaerobacterium saccharolyticum.Biotechnology for biofuels · 2013Article
- Direct conversion of xylan to ethanol by recombinant Saccharomyces cerevisiae strains displaying an engineered minihemicellulosome.Applied and environmental microbiology · 2012Article
- Scaffoldin modules serving as "cargo" domains to promote the secretion of heterologous cellulosomal cellulases by Clostridium acetobutylicum.Applied and environmental microbiology · 2011Article
- The issue of secretion in heterologous expression of Clostridium cellulolyticum cellulase-encoding genes in Clostridium acetobutylicum ATCC 824.Applied and environmental microbiology · 2011Article
- Yeast surface display of trifunctional minicellulosomes for simultaneous saccharification and fermentation of cellulose to ethanol.Applied and environmental microbiology · 2010Article
- Carbohydrate binding modules: biochemical properties and novel applications.Microbiology and molecular biology reviews : MMBR · 2006Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene man5K encoding the mannanase Man5K from Clostridium cellulolyticum was cloned alone or as an operon with the gene cipC1 encoding a truncated scaffoldin (miniCipC1) of the same origin in the solventogenic Clostridium acetobutylicum. The expression of the heterologous gene(s) was under the control of a weakened thiolase promoter Pthl. The recombinant strains of the solventogenic bacterium were both found to secrete active Man5K in the range of milligrams per liter. In the case of the strain expressing only man5K, a large fraction of the recombinant enzyme was truncated and lost the N-terminal dockerin domain, but it remained active towards galactomannan. When man5K was coexpressed with cipC1 in C. acetobutylicum, the recombinant strain secreted almost exclusively full-length mannanase, which bound to the scaffoldin miniCipC1, thus showing that complexation to the scaffoldin stabilized the enzyme. The secreted heterologous complex was found to be functional: it binds to crystalline cellulose via the carbohydrate binding module of the miniscaffoldin, and the complexed mannanase is active towards galactomannan. Taken together, these data show that C. acetobutylicum is a suitable host for the production, assembly, and secretion of heterologous minicellulosomes.
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