ArticleApplied and environmental microbiology2005
Efficient DNA fingerprinting of Clostridium botulinum types A, B, E, and F by amplified fragment length polymorphism analysis.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Low-level colonization of methicillin-resistant Staphylococcus aureus in pigs is maintained by slowly evolving, closely related strains in Finnish pig farms.Acta veterinaria Scandinavica · 2022Article
- Identification of foodborne pathogenic bacteria using confocal Raman microspectroscopy and chemometrics.Frontiers in microbiology · 2022Article
- Pan-Genomic Analysis ofToxins · 2020Article
- Comparative genomic analyses reveal broad diversity in botulinum-toxin-producing Clostridia.BMC genomics · 2016Article
- Clostridium botulinum Group II Isolate Phylogenomic Profiling Using Whole-Genome Sequence Data.Applied and environmental microbiology · 2015Article
- Historical and current perspectives on Clostridium botulinum diversity.Research in microbiology · 2015Review
- Mass Spectrometric Identification and Differentiation of Botulinum Neurotoxins through Toxin Proteomics.Reviews in analytical chemistry · 2013Article
- Genetic diversity of the flagellin genes of Clostridium botulinum groups I and II.Applied and environmental microbiology · 2013Article
- Plasmid-borne type E neurotoxin gene clusters in Clostridium botulinum strains.Applied and environmental microbiology · 2013Article
- Genetic diversity among Clostridium botulinum strains harboring bont/A2 and bont/A3 genes.Applied and environmental microbiology · 2012Article
- De novo subtype and strain identification of botulinum neurotoxin type B through toxin proteomics.Analytical and bioanalytical chemistry · 2012Article
- Exploring genomic diversity inThe botulinum journal · 2012Article
- Article
- Analysis of Clostridium botulinum serotype E strains by using multilocus sequence typing, amplified fragment length polymorphism, variable-number tandem-repeat analysis, and botulinum neurotoxin gene sequencing.Applied and environmental microbiology · 2011Article
- Clostridium botulinum group I strain genotyping by 15-locus multilocus variable-number tandem-repeat analysis.Journal of clinical microbiology · 2011Article
- Article
- Comparative genomic hybridization analysis of two predominant Nordic group I (proteolytic) Clostridium botulinum type B clusters.Applied and environmental microbiology · 2009Article
- Quantitative real-time reverse transcription-PCR analysis reveals stable and prolonged neurotoxin cluster gene activity in a Clostridium botulinum type E strain at refrigeration temperature.Applied and environmental microbiology · 2008Article
- Differentiation of Clostridium botulinum serotype A strains by multiple-locus variable-number tandem-repeat analysis.Applied and environmental microbiology · 2008Article
- Sequencing the botulinum neurotoxin gene and related genes in Clostridium botulinum type E strains reveals orfx3 and a novel type E neurotoxin subtype.Journal of bacteriology · 2007Article
Corrections and comments
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Amplified fragment length polymorphism (AFLP) analysis was applied to characterize 33 group I and 37 group II Clostridium botulinum strains. Four restriction enzyme and 30 primer combinations were screened to tailor the AFLP technique for optimal characterization of C. botulinum. The enzyme combination HindIII and HpyCH4IV, with primers having one selective nucleotide apiece (Hind-C and Hpy-A), was selected. AFLP clearly differentiated between C. botulinum groups I and II; group-specific clusters showed <10% similarity between proteolytic and nonproteolytic C. botulinum strains. In addition, group-specific fragments were detected in both groups. All strains studied were typeable by AFLP, and a total of 42 AFLP types were identified. Extensive diversity was observed among strains of C. botulinum type E, whereas group I had lower genetic biodiversity. These results indicate that AFLP is a fast, highly discriminating, and reproducible DNA fingerprinting method with excellent typeability, which, in addition to its suitability for typing at strain level, can be used for C. botulinum group identification.
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