ArticleNucleic acids research2005
Cadmium inhibits mismatch repair by blocking the ATPase activity of the MSH2-MSH6 complex.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 96 citations in OpenAlex.
- An Overview of Mycorrhiza in Pines: Research, Species, and Applications.Plants (Basel, Switzerland) · 2024Review
- MutSα and MutSβ as size-dependent cellular determinants for prime editing in human embryonic stem cells.Molecular therapy. Nucleic acids · 2023Article
- Protective effect assessment of Moringa oleifera against cadmium-induced toxicity in HCT116 and HEK293 cell lines.Environmental science and pollution research international · 2020Article
- Differential expression pattern of the proteome in response to cadmium stress based on proteomics analysis of wheat roots.BMC genomics · 2020Article
- Cell organelles as targets of mammalian cadmium toxicity.Archives of toxicology · 2020Review
- Chronic Exposure to Cadmium and Antioxidants Does Not Affect the Dynamics of Expanded CAG•CTG Trinucleotide Repeats in a Mouse Cell Culture System of Unstable DNA.Frontiers in cellular neuroscience · 2020Article
- Amelioration of toxicopathological effects of cadmium with silymarin and milk thistle in male Japanese quail (Coturnix japonica).Environmental science and pollution research international · 2019Article
- The mutagen and carcinogen cadmium is a high-affinity inhibitor of the zinc-dependent MutLα endonuclease.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
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- Cadmium(II) inhibition of human uracil-DNA glycosylase by catalytic water supplantation.Scientific reports · 2016Article
- Combined effects of cadmium and salinity on juvenile Takifugu obscurus: cadmium moderates salinity tolerance; salinity decreases the toxicity of cadmium.Scientific reports · 2016Article
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- Review
- Defects in base excision repair sensitize cells to manganese in S. cerevisiae.BioMed research international · 2013Article
- Cadmium accumulation in tissues of Sarotherodon melanotheron (Rüppel, 1852) from the Aby Lagoon system in Côte d'Ivoire.International journal of environmental research and public health · 2012Article
- A PLAC8-containing protein from an endomycorrhizal fungus confers cadmium resistance to yeast cells by interacting with Mlh3p.Nucleic acids research · 2011Article
- Alternative splicing of SLC39A14 in colorectal cancer is regulated by the Wnt pathway.Molecular & cellular proteomics : MCP · 2011Article
- Mechanism of cadmium-mediated inhibition of Msh2-Msh6 function in DNA mismatch repair.Biochemistry · 2009Article
- Discovery of ZIP transporters that participate in cadmium damage to testis and kidney.Toxicology and applied pharmacology · 2009Review
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 2 countries.
Funding
Abstract
Cadmium (Cd2+) is a known carcinogen that inactivates the DNA mismatch repair (MMR) pathway. In this study, we have tested the effect of Cd2+ exposure on the enzymatic activity of the mismatch binding complex MSH2-MSH6. Our results indicate that Cd2+ is highly inhibitory to the ATP binding and hydrolysis activities of MSH2-MSH6, and less inhibitory to its DNA mismatch binding activity. The inhibition of the ATPase activity appears to be dose and exposure time dependent. However, the inhibition of the ATPase activity by Cd2+ is prevented by cysteine and histidine, suggesting that these residues are essential for the ATPase activity and are targeted by Cd2+. A comparison of the mechanism of inhibition with N-ethyl maleimide, a sulfhydryl group inhibitor, indicates that this inhibition does not occur through direct inactivation of sulfhydryl groups. Zinc (Zn2+) does not overcome the direct inhibitory effect of Cd2+ on the MSH2-MSH6 ATPase activity in vitro. However, the increase in the mutator phenotype of yeast cells exposed to Cd2+ was prevented by excess Zn2+, probably by blocking the entry of Cd2+ into the cell. We conclude that the inhibition of MMR by Cd2+ is through the inactivation of the ATPase activity of the MSH2-MSH6 heterodimer, resulting in a dominant negative effect and causing a mutator phenotype.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.