ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2005
Trafficking of a ligand-receptor complex on the growth cones as an essential step for the uptake of nerve growth factor at the distal end of the axon: a single-molecule analysis.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 60 citations in OpenAlex.
- Studying structure and functions of cell membranes by single molecule biophysical techniques.Biophysics reports · 2021Article
- Dynamic organization of cortical actin filaments during the ooplasmic segregation of ascidianMolecular biology of the cell · 2021Article
- Control of neurite growth and guidance by an inhibitory cell-body signal.PLoS computational biology · 2018Article
- Drive the Car(go)s-New Modalities to Control Cargo Trafficking in Live Cells.Frontiers in molecular neuroscience · 2017Review
- Dissection of molecular assembly dynamics by tracking orientation and position of single molecules in live cells.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Precursor and mature NGF live tracking: one versus many at a time in the axons.Scientific reports · 2016Article
- Ligand-induced dynamics of neurotrophin receptors investigated by single-molecule imaging approaches.International journal of molecular sciences · 2015Review
- Real-time imaging of axonal transport of quantum dot-labeled BDNF in primary neurons.Journal of visualized experiments : JoVE · 2014Article
- Site-specific labeling of neurotrophins and their receptors via short and versatile peptide tags.PloS one · 2014Article
- Simultaneous profiling of 194 distinct receptor transcripts in human cells.Science signaling · 2013Article
- A novel method for producing mono-biotinylated, biologically active neurotrophic factors: an essential reagent for single molecule study of axonal transport.Journal of neuroscience methods · 2011Article
- Single-molecule spectroscopy and imaging of biomolecules in living cells.Analytical chemistry · 2010Article
- Statistical analysis of lateral diffusion and multistate kinetics in single-molecule imaging.Biophysical journal · 2009Article
- The trip of the tip: understanding the growth cone machinery.Nature reviews. Molecular cell biology · 2009Review
- The coming of age of axonal neurotrophin signaling endosomes.Journal of proteomics · 2009Review
- Tracking bio-molecules in live cells using quantum dots.Journal of biophotonics · 2008Review
- Modelling reaction kinetics inside cells.Essays in biochemistry · 2008Review
- One at a time, live tracking of NGF axonal transport using quantum dots.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Unc-51-like kinase 1/2-mediated endocytic processes regulate filopodia extension and branching of sensory axons.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Imaging single molecules in living cells for systems biology.Molecular systems biology · 2006Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The behavior of single molecules of neurotrophins on growth cones was observed by the use of the fluorescent conjugate of nerve growth factor (NGF), Cy3-NGF. After the application of 0.4 nm Cy3-NGF, chick dorsal root ganglion growth cones responded within 1 min of adding the stimulus by expanding their lamellipodia. Only 40 molecules of Cy3-NGF, which occupied <5% of the estimated total binding sites on a single growth cone, were required to initiate the motile responses. After binding to the high-affinity receptor, Cy3-NGF displayed lateral diffusion on the membrane of the growth cones with a diffusion constant of 0.3 microm2 s(-1). The behavior of Cy3-NGF was shifted to a one-directional rearward movement toward the central region of the growth cone. The one-directional movement of Cy3-NGF displayed the same rate as the rearward flow of actin, approximately 4 microm/min. This movement could be stopped by the application of the potent inhibitor of actin polymerization, latrunculin B. Molecules of Cy3-NGF were suggested to be internalized in the vicinity of the central region of the growth cone during this rearward trafficking, because Cy3-NGF remained in the growth cone after the growth cones had been exposed to an acidic surrounding medium: acidic medium causes the complete dissociation of Cy3-NGF from the receptors on the surface of growth cones. These results suggested that actin-driven trafficking of the NGF receptor complex is an essential step for the accumulation and endocytosis of NGF at the growth cone and for the retrograde transport of NGF toward the cell body.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.