ArticleMolecular genetics and genomics : MGG2005
Characterization of a mitochondrially targeted single-stranded DNA-binding protein in Arabidopsis thaliana.
Article in Molecular genetics and genomics : MGG, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- A record-setting mitogenome in the holoparasitic plant Balanophora yakushimensis accompanied by exceptional loss of organellar DNA repair and recombination genes.BMC biology · 2025Article
- The evolutionarily diverged single-stranded DNA-binding proteins SSB1/SSB2 differentially affect the replication, recombination and mutation of organellar genomes inPlant diversity · 2025Article
- Article
- Assembly of the Complete Mitochondrial Genome ofInternational journal of molecular sciences · 2023Article
- Telomerase Interaction Partners-Insight from Plants.International journal of molecular sciences · 2021Article
- Review
- Mitochondrial DNA Repair in anPlants (Basel, Switzerland) · 2020Article
- DNA Repair and the Stability of the Plant Mitochondrial Genome.International journal of molecular sciences · 2020Review
- Biolistic Approach for Transient Gene Expression Studies in Plants.Methods in molecular biology (Clifton, N.J.) · 2020Review
- Structure-Function Analysis Reveals the Singularity of Plant Mitochondrial DNA Replication Components: A Mosaic and Redundant System.Plants (Basel, Switzerland) · 2019Review
- Plant Organelle Genome Replication.Plants (Basel, Switzerland) · 2019Review
- Plant organellar DNA polymerases repair double-stranded breaks by microhomology-mediated end-joining.Nucleic acids research · 2019Article
- Minireview: DNA replication in plant mitochondria.Mitochondrion · 2014Review
- Enzymes involved in organellar DNA replication in photosynthetic eukaryotes.Frontiers in plant science · 2014Review
- Localization and phylogenetic analysis of enzymes related to organellar genome replication in the unicellular rhodophyte Cyanidioschyzon merolae.Genome biology and evolution · 2014Article
- A conserved lysine residue of plant Whirly proteins is necessary for higher order protein assembly and protection against DNA damage.Nucleic acids research · 2012Article
- Identification of a soybean chloroplast DNA replication origin-binding protein.Plant molecular biology · 2011Article
- A conserved, Mg²+-dependent exonuclease degrades organelle DNA during Arabidopsis pollen development.The Plant cell · 2011Article
- Crystal structures of DNA-Whirly complexes and their role in Arabidopsis organelle genome repair.The Plant cell · 2010Article
- Epstein-Barr virus immediate-early protein Zta co-opts mitochondrial single-stranded DNA binding protein to promote viral and inhibit mitochondrial DNA replication.Journal of virology · 2008Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
A gene encoding a predicted mitochondrially targeted single-stranded DNA binding protein (mtSSB) was identified in the Arabidopsis thaliana genome sequence. This gene (At4g11060) codes for a protein of 201 amino acids, including a 28-residue putative mitochondrial targeting transit peptide. Protein sequence alignment shows high similarity between the mtSSB protein and single-stranded DNA binding proteins (SSB) from bacteria, including residues conserved for SSB function. Phylogenetic analysis indicates a close relationship between this protein and other mitochondrially targeted SSB proteins. The predicted targeting sequence was fused with the GFP coding region, and the organellar localization of the expressed fusion protein was determined. Specific targeting to mitochondria was observed in in-vitro import experiments and by transient expression of a GFP fusion construct in Arabidopsis leaves after microprojectile bombardment. The mature mtSSB coding region was overexpressed in Escherichia coli and the protein was purified for biochemical characterization. The purified protein binds single-stranded, but not double-stranded, DNA. MtSSB stimulates the homologous strand-exchange activity of E. coli RecA. These results indicate that mtSSB is a functional homologue of the E. coli SSB, and that it may play a role in mitochondrial DNA recombination.
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