ArticleMolecular genetics and genomics : MGG2005
Chloroplast DNA base substitutions: an experimental assessment.
Article in Molecular genetics and genomics : MGG, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 24 citations in OpenAlex.
- High-Resolution Chloroplast SNV Profiling of 409 Grapevine (International journal of molecular sciences · 2026Article
- Highly Iterated Palindromic Sequences (HIPs) and Their Relationship to DNA Methyltransferases.Life (Basel, Switzerland) · 2015Article
- Complete Arabis alpina chloroplast genome sequence and insight into its polymorphism.Meta gene · 2013Article
- High-throughput sequencing of three Lemnoideae (duckweeds) chloroplast genomes from total DNA.PloS one · 2011Article
- Microhomology-mediated and nonhomologous repair of a double-strand break in the chloroplast genome of Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Creation of a chloroplast microsatellite reporter for detection of replication slippage in Chlamydomonas reinhardtii.Eukaryotic cell · 2008Article
- Chlamydomonas reinhardtii: a convenient model system for the study of DNA repair in photoautotrophic eukaryotes.Current genetics · 2008Review
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An experimental assessment was carried out to determine directly the frequency and types of spontaneous base substitutions that occur in chloroplast DNA. A target site within the chloroplast 16S rRNA gene of the green alga Chlamydomonas reinhardtii was chosen for the assay. Mutations at this site were known to confer spectinomycin resistance and simultaneously result in the loss of an AatII cleavage site. In the experiments reported here, base substitutions at any individual base occurred at a frequency in the range of 0.9-11 per 10(9) viable cells plated. Four new mutations that confer resistance to spectinomycin were identified at the target site in the Chlamydomonas chloroplast 16S rRNA gene. When the relative rates of transition and transversion mutations were quantified, a bias toward transversions was observed. The prominence of A/T --> C/G transversions in the observed mutation spectrum suggests that oxidative damage may be the major cause of base substitution mutations within the chloroplast.
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