ArticleGenetics2005
Evidence that Spt10 and Spt21 of Saccharomyces cerevisiae play distinct roles in vivo and functionally interact with MCB-binding factor, SCB-binding factor and Snf1.
Article in Genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 28 citations in OpenAlex.
- Decoupled transcript and protein concentrations ensure histone homeostasis in different nutrients.The EMBO journal · 2024Article
- DNA damage response activates respiration and thereby enlarges dNTP pools to promote cell survival in budding yeast.The Journal of biological chemistry · 2019Article
- Regulation of DNA replication-coupled histone gene expression.Oncotarget · 2017Review
- Regulation of histone gene transcription in yeast.Cellular and molecular life sciences : CMLS · 2014Review
- Cell-cycle perturbations suppress the slow-growth defect of spt10Δ mutants in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2013Article
- Regulation of histone gene expression in budding yeast.Genetics · 2012Review
- The mitotic Clb cyclins are required to alleviate HIR-mediated repression of the yeast histone genes at the G1/S transition.Biochimica et biophysica acta · 2012Article
- Insight into the mechanism of nucleosome reorganization from histone mutants that suppress defects in the FACT histone chaperone.Genetics · 2011Article
- Activator-independent transcription of Snf1-dependent genes in mutants lacking histone tails.Molecular microbiology · 2011Article
- Spt10 and Swi4 control the timing of histone H2A/H2B gene activation in budding yeast.Molecular and cellular biology · 2011Article
- A new fluorescence-based method identifies protein phosphatases regulating lipid droplet metabolism.PloS one · 2010Article
- Inferring Transcriptional Interactions by the Optimal Integration of ChIP-chip and Knock-out Data.Bioinformatics and biology insights · 2009Article
- The T body, a new cytoplasmic RNA granule in Saccharomyces cerevisiae.Molecular and cellular biology · 2008Article
- Identification of Rkr1, a nuclear RING domain protein with functional connections to chromatin modification in Saccharomyces cerevisiae.Molecular and cellular biology · 2007Article
- Cooperative binding of the yeast Spt10p activator to the histone upstream activating sequences is mediated through an N-terminal dimerization domain.Nucleic acids research · 2007Article
- Histone H3 Ser10 phosphorylation-independent function of Snf1 and Reg1 proteins rescues a gcn5- mutant in HIS3 expression.Molecular and cellular biology · 2005Article
- Global regulation by the yeast Spt10 protein is mediated through chromatin structure and the histone upstream activating sequence elements.Molecular and cellular biology · 2005Article
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2 authors at 1 institution in 1 country.
Funding
Abstract
Mutations in SPT10 and SPT21 of Saccharomyces cerevisiae have been previously shown to cause two prominent mutant phenotypes: (1) defects in transcription of particular histone genes and (2) suppression of Ty and delta-insertion mutations (Spt(-) phenotype). The requirement for Spt10 and Spt21 for transcription of particular histone genes suggested that they may interact with two factors previously shown to be present at histone loci, SBF (Swi4 and Swi6) and MBF (Mbp1 and Swi6). Therefore, we have studied swi4Delta, mbp1Delta, and swi6Delta mutants with respect to histone gene transcription and for interactions with spt10Delta and spt21Delta. Our results suggest that MBF and SBF play only modest roles in activation of histone gene transcription. In addition, we were surprised to find that swi4Delta, mbp1Delta, and swi6Delta mutations suppress the spt21Delta Spt(-) phenotype, but not the spt21Delta defect in histone gene transcription. In contrast, both swi4Delta and mbp1Delta cause lethality when combined with spt10Delta. To learn more about mutations that can suppress the spt21Delta Spt(-) phenotype, we performed a genetic screen and identified spt21Delta suppressors in seven additional genes. Three of these spt21Delta suppressors also cause lethality when combined with spt10Delta. Analysis of one spt21Delta suppressor, reg1, led to the finding that hyperactivation of Snf1 kinase, as caused by reg1Delta, suppresses the Spt(-) phenotype of spt21Delta. Taken together, these genetic interactions suggest distinct roles for Spt21 and Spt10 in vivo that are sensitive to multiple perturbations in transcription networks.
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