ArticleGenetics2005
Revisiting purine-histidine cross-pathway regulation in Saccharomyces cerevisiae: a central role for a small molecule.
Article in Genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed, 100 citations in OpenAlex.
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- Tetrahydrofolate levels influence 2-aminoacrylate stress inJournal of bacteriology · 2024Article
- MOB rules: Antibiotic Exposure Reprograms Metabolism to MobilizebioRxiv : the preprint server for biology · 2024Article
- Purine metabolism in plant pathogenic fungi.Frontiers in microbiology · 2024Review
- Improved production of TaxolMicrobial cell factories · 2023Article
- A Caenorhabditis elegans model of adenylosuccinate lyase deficiency reveals neuromuscular and reproductive phenotypes of distinct etiology.Molecular genetics and metabolism · 2023Article
- Ubiquitin Degradation of the AICAR Transformylase/IMP Cyclohydrolase Ade16 Regulates the Sexual Reproduction ofJournal of fungi (Basel, Switzerland) · 2023Article
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- In silico-guided metabolic engineering of Bacillus subtilis for efficient biosynthesis of purine nucleosides by blocking the key backflow nodes.Biotechnology for biofuels and bioproducts · 2022Article
- Purine Auxotrophic Starvation Evokes Phenotype Similar to Stationary Phase Cells in Budding Yeast.Journal of fungi (Basel, Switzerland) · 2021Article
- Metabolic and physiological responses to progressive drought stress in bread wheat.Scientific reports · 2020Article
- Reconstruction and analysis of genome-scale metabolic model of weak Crabtree positive yeast Lachancea kluyveri.Scientific reports · 2020Article
- Application of simultaneous selective pressures slows adaptation.Evolutionary applications · 2020Article
- Mutational Analysis of N-Ethyl-N-Nitrosourea (ENU) in the Fission YeastG3 (Bethesda, Md.) · 2020Article
- Selection markers for transformation of the sequenced reference monokaryon Okayama 7/#130 and homokaryon AmutBmut ofFungal biology and biotechnology · 2020Article
- Construction and characterization of centromeric plasmids for Komagataella phaffii using a color-based plasmid stability assay.PloS one · 2020Article
- Yeast glutaredoxin, GRX4, functions as a glutathione S-transferase required for red ade pigment formation inJournal of biosciences · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Because some metabolic intermediates are involved in more than one pathway, crosstalk between pathways is crucial to maintaining homeostasis. AMP and histidine biosynthesis pathways are coregulated at the transcriptional level in response to adenine availability. 5'-Phosphoribosyl-4-carboxamide-5-aminoimidazole (AICAR), a metabolic intermediate at the crossroads between these two pathways, is shown here to be critical for activation of the transcriptional response in the absence of adenine. In this study, we show that both AMP and histidine pathways significantly contribute to AICAR synthesis. Furthermore, we show that upregulation of the histidine pathway clearly interferes with regulation of the AMP pathway, thus providing an explanation for the regulatory crosstalk between these pathways. Finally, we revisit the histidine auxotrophy of ade3 or ade16 ade17 mutants. Interestingly, overexpression of PMU1, encoding a potential phosphomutase, partially suppresses the histidine requirement of an ade3 ade16 ade17 triple mutant, most probably by reducing the level of AICAR in this mutant. Together our data clearly establish that AICAR is not just a metabolic intermediate but also acts as a true regulatory molecule.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.