ArticleJournal of bacteriology2005
Regulating pilin expression reveals a threshold for S motility in Myxococcus xanthus.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Combinatorial control of type IVa pili formation by the four polarized regulators MglA, SgmX, FrzS, and SopA.Journal of bacteriology · 2024Article
- Article
- The PilT retraction ATPase promotes both extension and retraction of the MSHA type IVa pilus in Vibrio cholerae.PLoS genetics · 2022Article
- The σScientific reports · 2021Article
- CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus.Microbial cell factories · 2018Article
- Colony Expansion of Socially Motile Myxococcus xanthus Cells Is Driven by Growth, Motility, and Exopolysaccharide Production.PLoS computational biology · 2016Article
- MasABK proteins interact with proteins of the type IV pilin system to affect social motility of Myxococcus xanthus.PloS one · 2013Article
- Type IV pilin proteins: versatile molecular modules.Microbiology and molecular biology reviews : MMBR · 2012Review
- Two systems for conditional gene expression in Myxococcus xanthus inducible by isopropyl-β-D-thiogalactopyranoside or vanillate.Journal of bacteriology · 2012Article
- Comprehensive set of integrative plasmid vectors for copper-inducible gene expression in Myxococcus xanthus.Applied and environmental microbiology · 2012Article
- BacM, an N-terminally processed bactofilin of Myxococcus xanthus, is crucial for proper cell shape.Molecular microbiology · 2011Article
- The chaperone ClpX stimulates expression of Staphylococcus aureus protein A by Rot dependent and independent pathways.PloS one · 2010Article
- Myxobacteria, polarity, and multicellular morphogenesis.Cold Spring Harbor perspectives in biology · 2010Review
- PilA localization affects extracellular polysaccharide production and fruiting body formation in Myxococcus xanthus.Molecular microbiology · 2010Article
- Genetic analysis of the regulation of type IV pilus function by the Chp chemosensory system of Pseudomonas aeruginosa.Journal of bacteriology · 2010Article
- A vitamin B12-based system for conditional expression reveals dksA to be an essential gene in Myxococcus xanthus.Journal of bacteriology · 2009Article
- The mosaic genome of Anaeromyxobacter dehalogenans strain 2CP-C suggests an aerobic common ancestor to the delta-proteobacteria.PloS one · 2008Article
- PilB and PilT are ATPases acting antagonistically in type IV pilus function in Myxococcus xanthus.Journal of bacteriology · 2008Article
- Regulation of type IV fimbrial biogenesis in Dichelobacter nodosus.Journal of bacteriology · 2006Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
An isopropyl-beta-D-thiogalactopyranoside (IPTG)-inducible promoter was constructed in Myxococcus xanthus. The single-copy pilA gene encodes pilin, the monomer unit of M. xanthus type IV pili. To vary the level of pilA expression, we cloned its promoter in front of the lac operator, and a plasmid containing the construct was inserted into the chromosome of a DeltapilA strain. Induction of pilin expression increased smoothly as the dose of IPTG added to the culture was increased. IPTG-induced pilin rescued S motility of the DeltapilA strain to wild-type levels. The rate of S-motile swarming was found to be proportional to the number of pili (shear-sensitive pilin) produced rather than to the level of total pilin. In fact, S motility was not rescued until the total level of pilin was more than 50% of the wild-type level. This observation implies that a threshold concentration of pilin must be exceeded before the shear-sensitive material (pili) is polymerized in M. xanthus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.