ArticleMolecular and cellular biology2005
Octamer and Sox elements are required for transcriptional cis regulation of Nanog gene expression.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 186 papers.
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186 citing papers in PubMed, 487 citations in OpenAlex.
- Systematic identification of Oct4 transcriptional targets in embryonic stem cells using the auxin-inducible degron system and nascent RNA sequencing.Cell regeneration (London, England) · 2025Article
- Live imaging endogenous transcription factor dynamics reveals mechanisms of epiblast and primitive endoderm fate segregation.Current biology : CB · 2025Article
- Uncovering candidate Nanog-Helper genes in early mouse embryo differentiation using differential entropy and network inference.Scientific reports · 2025Article
- Transcriptome Analysis Suggested Striking Transition Around the End of Epiboly in the Gene Regulatory Network Downstream of the Oct4-Type POU Gene in Zebrafish Embryos.Development, growth & differentiation · 2025Article
- A live single-cell reporter system reveals drug-induced plasticity of a cancer stem cell-like population in cholangiocarcinoma.Scientific reports · 2024Article
- Research progress of nanog gene in fish.Molecular genetics and genomics : MGG · 2024Review
- Txnip regulates the Oct4-mediated pluripotency circuitry via metabolic changes upon differentiation.Cellular and molecular life sciences : CMLS · 2024Article
- A suboptimal OCT4-SOX2 binding site facilitates the naïve-state specific function of a Klf4 enhancer.PloS one · 2024Article
- AKT1 induces Nanog promoter in a SUMOylation-dependent manner in different pluripotent contexts.BMC research notes · 2023Article
- 6-Gingerol Improves In Vitro Porcine Embryo Development by Reducing Oxidative Stress.Animals : an open access journal from MDPI · 2023Article
- LIN28 and histone H3K4 methylase induce TLR4 to generate tumor-initiating stem-like cells.iScience · 2023Article
- Epigenetic Regulation of Driver Genes in Testicular Tumorigenesis.International journal of molecular sciences · 2023Review
- Regulation of Oct4 in stem cells and neural crest cells.Birth defects research · 2022Review
- Characterization of the Nanog gene involved in the gonadal development in pearlscale angelfish (Centropyge vrolikii).Fish physiology and biochemistry · 2022Article
- L-Proline Supplementation Drives Self-Renewing Mouse Embryonic Stem Cells to a Partially Primed Pluripotent State: The Early Primitive Ectoderm-Like Cell.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Retina stem cells, hopes and obstacles.World journal of stem cells · 2021Review
- Auxin-degron system identifies immediate mechanisms of OCT4.Stem cell reports · 2021Article
- TFEB regulates pluripotency transcriptional network in mouse embryonic stem cells independent of autophagy-lysosomal biogenesis.Cell death & disease · 2021Article
- Enhancer grammar in development, evolution, and disease: dependencies and interplay.Developmental cell · 2021Review
- Differential transcriptional regulation of the NANOG gene in chicken primordial germ cells and embryonic stem cells.Journal of animal science and biotechnology · 2021Article
126 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 2 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pluripotential cell-specific gene Nanog encodes a homeodomain-bearing transcription factor required for maintaining the undifferentiated state of stem cells. However, the molecular mechanisms that regulate Nanog gene expression are largely unknown. To address this important issue, we used luciferase assays to monitor the relative activities of deletion fragments from the 5'-flanking region of the gene. An adjacent pair of highly conserved Octamer- and Sox-binding sites was found to be essential for activating pluripotential state-specific gene expression. Furthermore, the 5'-end fragment encompassing the Octamer/Sox element was sufficient for inducing the proper expression of a green fluorescent protein reporter gene even in human embryonic stem (ES) cells. The potential of OCT4 and SOX2 to bind to this element was verified by electrophoretic mobility shift assays with extracts from F9 embryonal carcinoma cells and embryonic germ cells derived from embryonic day 12.5 embryos. However, in ES cell extracts, a complex of OCT4 with an undefined factor preferentially bound to the Octamer/Sox element. Thus, Nanog transcription may be regulated through an interaction between Oct4 and Sox2 or a novel pluripotential cell-specific Sox element-binding factor which is prominent in ES cells.
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