ArticleMolecular and cellular biology2005
Ligand-specific dynamics of the progesterone receptor in living cells and during chromatin remodeling in vitro.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
53 citing papers in PubMed, 110 citations in OpenAlex.
- Split Decisions in Hormone Signaling: Distinct Roles for Progesterone Receptor Isoforms in Breast Cancer Biology.Endocrinology · 2025Review
- Structural proteomics defines a sequential priming mechanism for the progesterone receptor.Nature communications · 2025Article
- Dynamic switching of transcriptional regulators between two distinct low-mobility chromatin states.Science advances · 2023Article
- 90 YEARS OF PROGESTERONE: Molecular mechanisms of progesterone receptor action on the breast cancer genome.Journal of molecular endocrinology · 2020Review
- Dynamic enhancer function in the chromatin context.Wiley interdisciplinary reviews. Systems biology and medicine · 2018Review
- The BRG1 ATPase of human SWI/SNF chromatin remodeling enzymes as a driver of cancer.Epigenomics · 2017Review
- Activity-Dependent Dynamics of the Transcription Factor of cAMP-Response Element Binding Protein in Cortical Neurons Revealed by Single-Molecule Imaging.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- DNA binding triggers tetramerization of the glucocorticoid receptor in live cells.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Divergent Binding and Transactivation by Two Related Steroid Receptors at the Same Response Element.The Journal of biological chemistry · 2016Article
- Rethinking progesterone regulation of female reproductive cyclicity.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Differential Regulation of Progesterone Receptor-Mediated Transcription by CDK2 and DNA-PK.Molecular endocrinology (Baltimore, Md.) · 2016Article
- Single-Molecule Imaging Reveals Dynamics of CREB Transcription Factor Bound to Its Target Sequence.Scientific reports · 2015Article
- Nucleosomes accelerate transcription factor dissociation.Nucleic acids research · 2014Article
- Distinctly different dynamics and kinetics of two steroid receptors at the same response elements in living cells.PloS one · 2014Article
- Eukaryotic transcriptional dynamics: from single molecules to cell populations.Nature reviews. Genetics · 2013Review
- Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation.Nucleic acids research · 2013Article
- Fluorescent ligand for human progesterone receptor imaging in live cells.Bioconjugate chemistry · 2013Article
- High-throughput RNA FISH analysis by imaging flow cytometry reveals that pioneer factor Foxa1 reduces transcriptional stochasticity.PloS one · 2013Article
- A benchmark for chromatin binding measurements in live cells.Nucleic acids research · 2012Article
- Complex dynamics of transcription regulation.Biochimica et biophysica acta · 2012Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Progesterone receptor (PR), a member of the nuclear receptor superfamily, is a key regulator of several processes in reproductive function. We have studied the dynamics of the interaction of PR with a natural target promoter in living cells through the use of fluorescence recovery after photobleaching (FRAP) analysis and also have characterized the dynamics of the interaction of PR with the mouse mammary tumor virus (MMTV) promoter reconstituted into chromatin in vitro. In photobleaching experiments, PR in the presence of the agonist R5020 exhibits rapid exchange with the MMTV promoter in living cells. Two PR antagonists, RU486 and ZK98299, have opposite effects on receptor dynamics in vivo. In the presence of RU486, PR binds to the promoter and is exchanged more slowly than the agonist-activated receptor. In contrast, PR bound to ZK98299 is not localized to the promoter and exhibits higher mobility in the nucleoplasm than the agonist-bound receptor. Significantly, PR bound to R5020 or RU486 can recruit the SWI/SNF chromatin remodeling complex to the promoter, but PR activated with ZK98299 cannot. Furthermore, we found ligand-specific active displacement of PR from the MMTV promoter during chromatin remodeling in vitro and conclude that the interaction of PR with chromatin is highly dynamic both in vivo and in vitro. We propose that factor displacement during chromatin remodeling is an important component of receptor mobility and that ligand-specific interactions with remodeling complexes can strongly influence receptor nuclear dynamics and rates of exchange with chromatin in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.