ArticleMolecular and cellular biology2005
RNA interference (RNAi)-dependent and RNAi-independent association of the Chp1 chromodomain protein with distinct heterochromatic loci in fission yeast.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Pathways of rDNA copy number homeostasis in Schizosaccharomyces pombe.G3 (Bethesda, Md.) · 2026Article
- Nucleosome remodeler exclusion by histone deacetylation enforces heterochromatic silencing and epigenetic inheritance.Molecular cell · 2024Article
- Impact of 1,6-hexanediol on Schizosaccharomyces pombe genome stability.G3 (Bethesda, Md.) · 2023Article
- The molecular basis of heterochromatin assembly and epigenetic inheritance.Molecular cell · 2023Review
- Histone deacetylation primes self-propagation of heterochromatin domains to promote epigenetic inheritance.Nature structural & molecular biology · 2022Article
- piRNA- and siRNA-mediated transcriptional repression in Drosophila, mice, and yeast: new insights and biodiversity.EMBO reports · 2021Review
- Subtelomeric Chromatin in the Fission YeastMicroorganisms · 2021Review
- RNA-induced initiation of transcriptional silencing (RITS) complex structure and function.RNA biology · 2019Review
- RNAi-dependent heterochromatin assembly in fission yeast Schizosaccharomyces pombe requires heat-shock molecular chaperones Hsp90 and Mas5.Epigenetics & chromatin · 2018Article
- Article
- Ser7 of RNAPII-CTD facilitates heterochromatin formation by linking ncRNA to RNAi.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The Conserved RNA Binding Cyclophilin, Rct1, Regulates Small RNA Biogenesis and Splicing Independent of Heterochromatin Assembly.Cell reports · 2017Article
- Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB.Molecular biology of the cell · 2017Article
- SNF2 Family Protein Fft3 Suppresses Nucleosome Turnover to Promote Epigenetic Inheritance and Proper Replication.Molecular cell · 2017Article
- Nucleation and spreading of a heterochromatic domain in fission yeast.Nature communications · 2016Article
- The Chp1 chromodomain binds the H3K9me tail and the nucleosome core to assemble heterochromatin.Cell discovery · 2016Article
- RNAi and heterochromatin assembly.Cold Spring Harbor perspectives in biology · 2015Review
- The Mi-2 homolog Mit1 actively positions nucleosomes within heterochromatin to suppress transcription.Molecular and cellular biology · 2014Article
- Sir2 is required for Clr4 to initiate centromeric heterochromatin assembly in fission yeast.The EMBO journal · 2013Article
- Distinct roles for Sir2 and RNAi in centromeric heterochromatin nucleation, spreading and maintenance.The EMBO journal · 2013Article
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Authors and funding
5 authors.
Funding
Abstract
The establishment of centromeric heterochromatin in the fission yeast Schizosaccharomyces pombe is dependent on the RNA interference (RNAi) pathway. Dicer cleaves centromeric transcripts to produce short interfering RNAs (siRNAs) that actively recruit components of heterochromatin to centromeres. Both centromeric siRNAs and the heterochromatin component Chp1 are components of the RITS (RNA-induced initiation of transcriptional gene silencing) complex, and the association of RITS with centromeres is linked to Dicer activity. In turn, centromeric binding of RITS promotes Clr4-mediated methylation of histone H3 lysine 9 (K9), recruitment of Swi6, and formation of heterochromatin. Similar to centromeres, the mating type locus (Mat) is coated in K9-methylated histone H3 and is bound by Swi6. Here we report that Chp1 associates with the mating type locus and telomeres and that Chp1 localization to heterochromatin depends on its chromodomain and the C-terminal domain of the protein. Another protein component of the RITS complex, Tas3, also binds to Mat and telomeres. Tas3 interacts with Chp1 through the C-terminal domain of Chp1, and this interaction is necessary for Tas3 stability. Interestingly, in cells lacking the Argonaute (Ago1) protein component of the RITS complex, or lacking Dicer (and hence siRNAs), Chp1 and Tas3 can still bind to noncentromeric loci, although their association with centromeres is lost. Thus, Chp1 and Tas3 exist as an Ago1-independent subcomplex that associates with noncentromeric heterochromatin independently of the RNAi pathway.
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