ArticleMolecular and cellular biology2005
Performance of genomic bordering elements at predefined genomic loci.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 74 citations in OpenAlex.
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- Site-specific chromosomal gene insertion: Flp recombinase versus Cas9 nuclease.Scientific reports · 2017Article
- Article
- Impact of using different promoters and matrix attachment regions on recombinant protein expression level and stability in stably transfected CHO cells.Molecular biotechnology · 2015Article
- Persistence of non-viral vector mediated RPE65 expression: case for viability as a gene transfer therapy for RPE-based diseases.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- A review of therapeutic prospects of non-viral gene therapy in the retinal pigment epithelium.Biomaterials · 2013Review
- Cis-regulation of microRNA expression by scaffold/matrix-attachment regions.Nucleic acids research · 2011Article
- Development of S/MAR minicircles for enhanced and persistent transgene expression in the mouse liver.Journal of molecular medicine (Berlin, Germany) · 2011Article
- Use of human MAR elements to improve retroviral vector production.Gene therapy · 2011Article
- Strategies to insulate lentiviral vector-expressed transgenes.Methods in molecular biology (Clifton, N.J.) · 2010Article
- Differential nuclear scaffold/matrix attachment marks expressed genes.Human molecular genetics · 2009Article
- Simultaneous excision of two transgene flanking sequences and resolution of complex integration loci.Plant molecular biology · 2009Article
- Maintenance of a functional higher order chromatin structure: The role of the nuclear matrix in normal and disease states.Gene therapy & molecular biology · 2009Article
- Protection against telomeric position effects by the chicken cHS4 beta-globin insulator.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- A comparative study of S/MAR prediction tools.BMC bioinformatics · 2007Article
- Chromatin, gene silencing and HIV latency.Genome biology · 2007Review
- Identification, genome mapping, and CTCF binding of potential insulators within the FXYD5-COX7A1 locus of human chromosome 19q13.12.Mammalian genome : official journal of the International Mammalian Genome Society · 2006Article
- Recommended Method for Chromosome Exploitation: RMCE-based Cassette-exchange Systems in Animal Cell Biotechnology.Cytotechnology · 2006Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eukaryotic DNA is organized into chromatin domains that regulate gene expression and chromosome behavior. Insulators and/or scaffold-matrix attachment regions (S/MARs) mark the boundaries of these chromatin domains where they delimit enhancing and silencing effects from the outside. By recombinase-mediated cassette exchange (RMCE), we were able to compare these two types of bordering elements at a number of predefined genomic loci. Flanking an expression vector with either S/MARs or two copies of the non-S/MAR chicken hypersensitive site 4 insulator demonstrates that while these borders confer related expression characteristics at most loci, their effect on chromatin organization is clearly distinct. Our results suggest that the activity of bordering elements is most pronounced for the abundant class of loci with a low but negligible expression potential in the case of highly expressed sites. By the RMCE procedure, we demonstrate that expression parameters are not due to a potential targeting action of bordering elements, in the sense that a linked transgene is directed into a special class of loci. Instead, we can relate the observed transcriptional augmentation phenomena to their function as genomic insulators.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.