ArticleNucleic acids research2005
Molecular characterization of Drosophila NELF.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 56 citations in OpenAlex.
- TLR7 modulates glioblastoma progression through PI3K/AKT/mTOR pathway and immune microenvironment remodeling.Irish journal of medical science · 2026Article
- Chemical catalyst manipulating cancer epigenome and transcription.Nature communications · 2025Article
- Transcriptional elongation control in developmental gene expression, aging, and disease.Molecular cell · 2023Review
- Recent advances in the chromatin-based mechanism ofFrontiers in plant science · 2022Review
- NELF-A controls Drosophila healthspan by regulating heat-shock protein-mediated cellular protection and heterochromatin maintenance.Aging cell · 2021Article
- Acute NelfA knockdown restricts compensatory gene expression and precipitates ventricular dysfunction during cardiac hypertrophy.Journal of molecular and cellular cardiology · 2020Article
- NELF Regulates a Promoter-Proximal Step Distinct from RNA Pol II Pause-Release.Molecular cell · 2020Article
- NELFE promoted pancreatic cancer metastasis and the epithelial‑to‑mesenchymal transition by decreasing the stabilization of NDRG2 mRNA.International journal of oncology · 2019Article
- Collaborative repressive action of the antagonistic ETS transcription factors Pointed and Yan fine-tunes gene expression to confer robustness inDevelopment (Cambridge, England) · 2018Article
- Identification of Regions in the Spt5 Subunit of DRB Sensitivity-inducing Factor (DSIF) That Are Involved in Promoter-proximal Pausing.The Journal of biological chemistry · 2017Article
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- Translational Initiation at a Non-AUG Start Codon for Human and Mouse Negative Elongation Factor-B.PloS one · 2015Article
- Comprehensive analysis of RNA-protein interactions by high-throughput sequencing-RNA affinity profiling.Nature methods · 2014Article
- Tip110 protein binds to unphosphorylated RNA polymerase II and promotes its phosphorylation and HIV-1 long terminal repeat transcription.The Journal of biological chemistry · 2014Article
- Kinetic competition between elongation rate and binding of NELF controls promoter-proximal pausing.Molecular cell · 2013Article
- Extensive polymerase pausing during Drosophila axis patterning enables high-level and pliable transcription.Genes & development · 2013Article
- The chromatin remodeling and mRNA splicing functions of the Brahma (SWI/SNF) complex are mediated by the SNR1/SNF5 regulatory subunit.Nucleic acids research · 2012Article
- Genetic and genomic analyses of RNA polymerase II-pausing factor in regulation of mammalian transcription and cell growth.The Journal of biological chemistry · 2011Article
- RNA polymerase II stalling promotes nucleosome occlusion and pTEFb recruitment to drive immortalization by Epstein-Barr virus.PLoS pathogens · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
NELF and DSIF act together to inhibit transcription elongation in vitro, and are implicated in causing promoter proximal pausing on the hsp70 gene in Drosophila. Here, further characterization of Drosophila NELF is provided. Drosophila NELF has four subunits similar to subunits of human NELF. The amino acid sequences of NELF-B and NELF-D are highly conserved throughout their lengths, while NELF-A and NELF-E contain nonconserved regions inserted between conserved N- and C-terminal regions. Immunodepletion of NELF or DSIF from a nuclear extract desensitizes transcription in vitro to DRB. Immunodepletion of NELF also impairs promoter proximal pausing on the hsp70 promoter in vitro without affecting initiation. Chromatin immunoprecipitation analyses detect NELF at the promoters of the hsp70 and beta1-tubulin genes where promoter proximal pausing has been previously detected. Heat shock induction of hsp70 results in a marked decrease in NELF at the hsp70 promoter. Immunofluorescence analysis of polytene chromosomes shows extensive colocalization of the NELF-B and NELF-D subunits at hundreds of interbands. Neither subunit appears to be recruited to puffs. These results provide a foundation for genetic and biochemical analysis of NELF in Drosophila.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.