ArticleNucleic acids research2005
An efficient system to establish multiple embryonic stem cell lines carrying an inducible expression unit.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 107 citations in OpenAlex.
- MGA directly recruits SETDB1/ATF7IP for histone H3K9me3 mark on meiosis-related genes in mouse embryonic stem cells.iScience · 2025Article
- Odz4 upregulates SAN-specific genes to promote differentiation into cardiac pacemaker-like cells.FEBS letters · 2025Article
- Enhancement of physiology via adaptive transcription.Pflugers Archiv : European journal of physiology · 2025Review
- Repression of germline genes by PRC1.6 and SETDB1 in the early embryo precedes DNA methylation-mediated silencing.Nature communications · 2021Article
- Identification of germ cell-specific Mga variant mRNA that promotes meiosis via impediment of a non-canonical PRC1.Scientific reports · 2021Article
- A transcriptomic study of Williams-Beuren syndrome associated genes in mouse embryonic stem cells.Scientific data · 2019Article
- Multiplexed Cas9 targeting reveals genomic location effects and gRNA-based staggered breaks influencing mutation efficiency.Nature communications · 2019Article
- The nucleosome remodeling and deacetylase complex protein CHD4 regulates neural differentiation of mouse embryonic stem cells by down-regulating p53.The Journal of biological chemistry · 2019Article
- PRDM14 Drives OCT3/4 Recruitment via Active Demethylation in the Transition from Primed to Naive Pluripotency.Stem cell reports · 2016Article
- Determination of local chromatin composition by CasID.Nucleus (Austin, Tex.) · 2016Article
- Generation and gene expression profiling of 48 transcription-factor-inducible mouse embryonic stem cell lines.Scientific reports · 2016Article
- Zscan4 Is Activated after Telomere Shortening in Mouse Embryonic Stem Cells.Stem cell reports · 2016Article
- Non-neural and cardiac differentiating properties of Tbx6-expressing mouse embryonic stem cells.Regenerative therapy · 2016Article
- Sox7 is dispensable for primitive endoderm differentiation from mouse ES cells.BMC developmental biology · 2015Article
- A modular open platform for systematic functional studies under physiological conditions.Nucleic acids research · 2015Article
- Generation of stomach tissue from mouse embryonic stem cells.Nature cell biology · 2015Article
- Integrative Analysis of the Acquisition of Pluripotency in PGCs Reveals the Mutually Exclusive Roles of Blimp-1 and AKT Signaling.Stem cell reports · 2015Article
- A novel autoregulatory loop between the Gcn2-Atf4 pathway and (L)-Proline [corrected] metabolism controls stem cell identity.Cell death and differentiation · 2015Article
- TBX1 Represses Vegfr2 Gene Expression and Enhances the Cardiac Fate of VEGFR2+ Cells.PloS one · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The growing use of mouse embryonic stem (ES) cells in research emphasizes their importance in studies of molecular mechanisms that maintain pluripotency and direct cellular differentiation. Although systems for regulatable transgene expression are essential for fine analysis of cellular processes at the molecular level, a strategy for the establishment of multiple ES cell lines carrying any of these systems has not yet been described. Here, we report our development of the ROSA-TET system, an effective system for the establishment of multiple ES cell lines carrying a tetracycline (Tc)-regulatable transgene at the Gt (ROSA)26asSor (ROSA26) locus. This system contains a knock-in step of a construct carrying both loxP and its mutant sequences into the ROSA26 locus, followed by a subsequent exchange step that introduces a cDNA to be Tc-regulated to the locus using the recombinase-mediated cassette exchange reaction. Both steps are demonstrated to give desired clones with high efficiency, suggesting that this system can be introduced readily into any ES cell lines, leading to the simultaneous establishment of multiple cell lines carrying different Tc-regulated cDNAs. We believe that use of this system will strongly accelerate molecular biological research using ES cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.