ArticleProceedings of the National Academy of Sciences of the United States of America2005
Three-dimensional reconstruction of the dynactin complex by single-particle image analysis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.
- Causal effects of blood cells on breast cancer: Evidence from bidirectional Mendelian randomization combined with meta-analysis.Medicine · 2025Pooled it
- Single-molecule imaging of stochastic interactions that drive dynein activation and cargo movement in cells.The Journal of cell biology · 2024Article
- Actin-capping protein regulates actomyosin contractility to maintain germline architecture in C. elegans.Development (Cambridge, England) · 2023Article
- Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathway.The Journal of cell biology · 2021Article
- Conformational diversity of dynactin sidearm and domain organization of its subunit p150.Molecular biology of the cell · 2020Article
- Structural organization of the dynein-dynactin complex bound to microtubules.Nature structural & molecular biology · 2015Article
- The structure of the dynactin complex and its interaction with dynein.Science (New York, N.Y.) · 2015Article
- Dynactin 3D structure: implications for assembly and dynein binding.Journal of molecular biology · 2014Article
- Dynamic actin gene family evolution in primates.BioMed research international · 2013Article
- The p25 subunit of the dynactin complex is required for dynein-early endosome interaction.The Journal of cell biology · 2011Article
- Arp11 affects dynein-dynactin interaction and is essential for dynein function in Aspergillus nidulans.Traffic (Copenhagen, Denmark) · 2008Article
- New insights into mechanism and regulation of actin capping protein.International review of cell and molecular biology · 2008Review
- The actin multigene family of Paramecium tetraurelia.BMC genomics · 2007Article
- Alanine scanning of Arp1 delineates a putative binding site for Jnm1/dynamitin and Nip100/p150Glued.Molecular biology of the cell · 2005Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Dynactin is a large complex of at least nine distinct proteins that co-complexes with cytoplasmic dynein within cells, where it plays a major role as a regulator of the motor's function. Owing to its large size and complexity, relatively little is known about dynactin's 3D structure or the structural basis of its function. Use of single-particle image analysis techniques has enabled us to produce the first 3D reconstruction of the dynactin complex, to a resolution of 3 nm. The actin-related protein (Arp) backbone of the filament has been clearly visualized. Fitting of models of the Arp backbone showed that it consists of 10 subunits. Additional mass, not part of the Arp backbone, was also seen. A preliminary fitting of the capping protein CapZ structure into our 3D reconstruction of the dynactin complex suggests that it is optimally placed to perform its proposed function as a stabilizer of the Arp1 backbone and gives clues as to likely interaction points between the capping protein and Arp subunits. The results provide the first detailed visualization of the dynactin complex and shed light on the mode of interaction between several of its constituent proteins and their possible functions.
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