ArticlePLoS biology2005
Cell-by-cell dissection of gene expression and chromosomal interactions reveals consequences of nuclear reorganization.
Article in PLoS biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 50 citations in OpenAlex.
- Polymorphic 3D genome architecture mediated by transposable elements.bioRxiv : the preprint server for biology · 2026Article
- DNA Double-Strand Breaks Affect Chromosomal Rearrangements during Methotrexate-Mediated Gene Amplification in Chinese Hamster Ovary Cells.Pharmaceutics · 2021Article
- Chromatin organization changes during the establishment and maintenance of the postmitotic state.Epigenetics & chromatin · 2017Article
- MeCP2_E1 N-terminal modifications affect its degradation rate and are disrupted by the Ala2Val Rett mutation.Human molecular genetics · 2017Article
- Article
- Perspectives: using polymer modeling to understand the formation and function of nuclear compartments.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2017Review
- Pivotal Impacts of Retrotransposon Based Invasive RNAs on Evolution.Frontiers in microbiology · 2017Review
- Review
- From Function to Phenotype: Impaired DNA Binding and Clustering Correlates with Clinical Severity in Males with Missense Mutations in MECP2.Scientific reports · 2016Article
- Gene repositioning within the cell nucleus is not random and is determined by its genomic neighborhood.Epigenetics & chromatin · 2015Article
- Reprogramming of fibroblast nuclei in cloned bovine embryos involves major structural remodeling with both striking similarities and differences to nuclear phenotypes of in vitro fertilized embryos.Nucleus (Austin, Tex.) · 2014Article
- Position-effect variegation, heterochromatin formation, and gene silencing in Drosophila.Cold Spring Harbor perspectives in biology · 2013Review
- Heterochromatin and gene positioning: inside, outside, any side?Chromosoma · 2012Article
- Heterochromatin as an incubator for pathology and treatment non-response: implication for neuropsychiatric illness.The pharmacogenomics journal · 2012Review
- 3D-Image analysis platform monitoring relocation of pluripotency genes during reprogramming.Nucleic acids research · 2011Article
- Stable transmission of reversible modifications: maintenance of epigenetic information through the cell cycle.Cellular and molecular life sciences : CMLS · 2011Review
- Perturbation analysis of heterochromatin-mediated gene silencing and somatic inheritance.PLoS genetics · 2010Article
- Gene positioning.Cold Spring Harbor perspectives in biology · 2010Review
- The molecular basis for stability of heterochromatin-mediated silencing in mammals.Epigenetics & chromatin · 2009Article
- Spatial allelic imbalance of BCL2 genes and chromosome 18 territories in nonneoplastic and neoplastic cervical squamous epithelium.European biophysics journal : EBJ · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The functional consequences of long-range nuclear reorganization were studied in a cell-by-cell analysis of gene expression and long-range chromosomal interactions in the Drosophila eye and eye imaginal disk. Position-effect variegation was used to stochastically perturb gene expression and probe nuclear reorganization. Variegating genes on rearrangements of Chromosomes X, 2, and 3 were probed for long-range interactions with heterochromatin. Studies were conducted only in tissues known to express the variegating genes. Nuclear structure was revealed by fluorescence in situ hybridization with probes to the variegating gene and heterochromatin. Gene expression was determined alternately by immunofluorescence against specific proteins and by eye pigment autofluorescence. This allowed cell-by-cell comparisons of nuclear architecture between cells in which the variegating gene was either expressed or silenced. Very strong correlations between heterochromatic association and silencing were found. Expressing cells showed a broad distribution of distances between variegating genes and their own centromeric heterochromatin, while silenced cells showed a very tight distribution centered around very short distances, consistent with interaction between the silenced genes and heterochromatin. Spatial and temporal analysis of interactions with heterochromatin indicated that variegating genes primarily associate with heterochromatin in cells that have exited the cell cycle. Differentiation was not a requirement for association, and no differences in association were observed between cell types. Thus, long-range interactions between distal chromosome regions and their own heterochromatin have functional consequences for the organism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.