Evidence map›Paper›PMID 15735741›Full record

ArticleOncogene2005

DIM stimulates IFNgamma gene expression in human breast cancer cells via the specific activation of JNK and p38 pathways.

Ling Xue, Gary L Firestone, Leonard F Bjeldanes

Abstract read
PubMed Publisher
In one paragraph

Article in Oncogene, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 1 pooled it
1.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 1 synthesis or guideline pooled it, 69 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Ling XueDepartment of Nutritional Sciences and Toxicology, University of California, 119 Morgan Hall, Berkeley, CA 94720-3104, USA.
Gary L Firestone
Leonard F Bjeldanes
University of California, Berkeley · US

Funding

REGULATION OF TUMOR ANGIOGENESIS BY DIETARY INDOLESR01CA069056 · NCI · UNIVERSITY OF CALIFORNIA BERKELEY · PI BJELDANES, LEONARD F · 1996 to 2004
$1.5M
Transcriptional &cell cycle control by dietary indolesR01CA102360 · NCI · UNIVERSITY OF CALIFORNIA BERKELEY · PI FIRESTONE, GARY L · 2004 to 2008
$1.3M
NCI NIH HHS CA102360NCI NIH HHS CA69056NCI NIH HHS R01 CA102360
6 · The paper itself

Abstract

3,3'-Diindolylmethane (DIM) is a promising anticancer agent derived from Brassica vegetables, but the mechanisms of DIM action are largely unknown. We have shown that DIM can upregulate the expression and stimulate the secretion of interferon-gamma (IFNgamma) in the human MCF-7 breast cancer cell line. This novel effect may provide important clues to explain the anticancer effects of DIM because it is well known that IFNgamma plays an important role in preventing the development of primary and transplanted tumors. Utilizing promoter deletions, we show here that the region between -108 and -36 bp in the IFNgamma promoter, which contains two conserved and essential regulatory elements, is required for DIM-induced IFNgamma expression. DIM activates both JNK and p38 pathways, induces the phosphorylation of c-Jun and ATF-2, and increases the binding of the homodimer or heterodimer of c-Jun/ATF-2 to the proximal AP-1.CREB-ATF-binding element. Moreover, studies with specific enzyme inhibitors showed that up-stream Ca2+-dependent kinase(s) is required for the inducing effects of DIM in MCF-7 cells. These results establish that DIM-induced IFNgamma expression in human breast tumor cells is mediated by activation of both JNK and p38 pathways, which is ultimately dependent on intracellular calcium signaling.

Indexed as

Activating Transcription Factor 2Base SequenceBreast NeoplasmsCell Line, TumorCyclic AMP Response Element-Binding ProteinDNA PrimersEnzyme ActivationGene Expression RegulationHumansIndolesInterferon-gammaJNK Mitogen-Activated Protein KinasesMAP Kinase Kinase 4Mitogen-Activated Protein Kinase Kinasesp38 Mitogen-Activated Protein KinasesProtein Binding3,3'-diindolylmethaneActivating Transcription Factor 2ATF2 protein, humanCyclic AMP Response Element-Binding ProteinDNA PrimersIndolesInterferon-gammaJNK Mitogen-Activated Protein KinasesMAP Kinase Kinase 4Mitogen-Activated Protein Kinase Kinasesp38 Mitogen-Activated Protein KinasesProto-Oncogene Proteins c-junTranscription Factors

Identifiers

PMID15735741
OpenAlexW1972735086

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.