ArticleJournal of clinical pathology2005
Immunohistochemical assays in prostatic biopsies processed in Bouin's fixative.
Article in Journal of clinical pathology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Improvement and development of immersion fixatives for enhanced tissue preservation in the whole eyeball specimens.The Journal of veterinary medical science · 2025Article
- Effects of biological fixation methods on stimulated Raman scattering microscopy signal.bioRxiv : the preprint server for biology · 2025Article
- Fish in focus: Navigating organ damage assessment through analytical avenues - A comprehensive review.Toxicology reports · 2024Review
- Quantifying mRNA in Highly Degraded Fixed Tissues by Nanostring Technology: A Comparative Study.Methods and protocols · 2024Article
- Effect of Fixatives and Fixation Period on Morphology and Immunohistochemistry of Feline Ovarian Tissue.Animals : an open access journal from MDPI · 2024Article
- The Anticonvulsant Effects of Baldrinal on Pilocarpine-Induced convulsion in Adult Male Mice.Molecules (Basel, Switzerland) · 2019Article
- Systematic comparison of tissue fixation with alternative fixatives to conventional tissue fixation with buffered formalin in a xenograft-based model.Virchows Archiv : an international journal of pathology · 2012Article
- Histology atlas of the developing mouse hepatobiliary system with emphasis on embryonic days 9.5-18.5.Toxicologic pathology · 2010Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
aimsTo investigate the problems involved in undertaking immunohistochemistry (IHC) and nuclear morphometry using Bouin's fixed prostate biopsies.
methodsArchival Bouin's fixed and formalin fixed, paraffin wax embedded prostatic biopsies were immunostained for three nuclear biomarkers (minichromosome maintenance protein 2 (MCM-2), p27, and Ki-67), one membrane localised biomarker (C-erb-B2), CD34, and alpha methylacyl-CoA racemase (AMACR). The quality of IHC staining was compared between tissues prepared separately in both fixatives. Feulgen staining was also performed on Bouin's fixed tissues to check its suitability for nuclear morphometry.
resultsMCM-2 staining was completely negative in Bouin's fixed tissues, whereas p27 showed more background and excess cytoplasmic staining in Bouin's fixed versus formalin fixed tissues. C-erb-B2 showed non-specific, strong luminal cell staining in the Bouin's fixed tissue. Feulgen staining was also very weak in Bouin's fixed tissue. However, Ki-67, AMACR, and CD34 worked equally well in Bouin's and formalin fixed tissues.
conclusionsBouin's fixed tissues may be unsuitable when subsequent IHC and morphometry are contemplated. An awareness of which antibodies are suitable for use in Bouin's fixed biopsies is essential.
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