ArticlePlant physiology2005
Proteomic identification of S-nitrosylated proteins in Arabidopsis.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 228 papers.
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Who cites it
228 citing papers in PubMed, 727 citations in OpenAlex.
- Multiomics Insights in Research of Nitric Oxide (NO) Donors in Plants upon Stress.International journal of molecular sciences · 2026Review
- Nitric Oxide Signaling in Photosynthetic Resilience: Linking Photoprotection and Carbon Gain in Tropical Crops.International journal of molecular sciences · 2026Review
- A Comparative Study on β-ODAP Level, Polyphenolic Content and Antioxidant Activity Across ThreeFood science & nutrition · 2026Article
- Linking redox and calcium signaling via thioredoxins.Journal of experimental botany · 2026Review
- Foliar L-arginine and seaweed extract mitigate salinity stress in Calendula officinalis L. by enhancing physiological and biochemical performance.BMC plant biology · 2026Article
- GSNOR mediates NO signaling to enhance salt tolerance in tobacco by regulating redox and photosynthesis.Photosynthesis research · 2026Article
- Integration of NO and HPlant communications · 2026Review
- From odor to order: unveiling the crucial role of hydrogen sulfide in plant life.Horticulture research · 2026Article
- The Central Role of GSNOR: Decoding Nitric Oxide Signaling for Crop Stress Tolerance.International journal of molecular sciences · 2025Review
- Enhancing rice crop resistance against brown plant hopper infestation through the foliar application of sodium nitroprusside.Plant molecular biology · 2025Article
- The Hidden Architects: Nitric Oxide and Redox Dynamics in Plant Stem Cell Homeostasis.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Nitric Oxide and Photosynthesis Interplay in Plant Interactions with Pathogens.International journal of molecular sciences · 2025Review
- Integrating Redox Proteomics and Computational Modeling to Decipher Thiol-Based Oxidative Post-Translational Modifications (oxiPTMs) in Plant Stress Physiology.International journal of molecular sciences · 2025Review
- Redox-signaling in innate immune memory: Similar mechanisms in animals/humans and plants.Redox biology · 2025Review
- Regulation of plant glycolysis and the tricarboxylic acid cycle by posttranslational modifications.The Plant journal : for cell and molecular biology · 2025Review
- Progress in Plant Nitric Oxide Studies: Implications for Phytopathology and Plant Protection.International journal of molecular sciences · 2025Review
- Nitro-fatty acids modulate germination onset through S-nitrosothiol metabolism.Plant physiology · 2025Article
- Assessing the role of exogenous NO on plants and microbial communities in soil.ISME communications · 2025Article
- Post-Translational Modifications to Cysteine Residues in Plant Proteins and Their Impact on the Regulation of Metabolism and Signal Transduction.International journal of molecular sciences · 2024Review
- Role of protein S-nitrosylation in plant growth and development.Plant cell reports · 2024Review
168 more citing papers are in PubMed but not listed here.
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although nitric oxide (NO) has grown into a key signaling molecule in plants during the last few years, less is known about how NO regulates different events in plants. Analyses of NO-dependent processes in animal systems have demonstrated protein S-nitrosylation of cysteine (Cys) residues to be one of the dominant regulation mechanisms for many animal proteins. For plants, the principle of S-nitrosylation remained to be elucidated. We generated S-nitrosothiols by treating extracts from Arabidopsis (Arabidopsis thaliana) cell suspension cultures with the NO-donor S-nitrosoglutathione. Furthermore, Arabidopsis plants were treated with gaseous NO to analyze whether S-nitrosylation can occur in the specific redox environment of a plant cell in vivo. S-Nitrosylated proteins were detected by a biotin switch method, converting S-nitrosylated Cys to biotinylated Cys. Biotin-labeled proteins were purified and analyzed using nano liquid chromatography in combination with mass spectrometry. We identified 63 proteins from cell cultures and 52 proteins from leaves that represent candidates for S-nitrosylation, including stress-related, redox-related, signaling/regulating, cytoskeleton, and metabolic proteins. Strikingly, many of these proteins have been identified previously as targets of S-nitrosylation in animals. At the enzymatic level, a case study demonstrated NO-dependent reversible inhibition of plant glyceraldehyde-3-phosphate dehydrogenase, suggesting that this enzyme could be affected by S-nitrosylation. The results of this work are the starting point for further investigation to get insight into signaling pathways and other cellular processes regulated by protein S-nitrosylation in plants.
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