ArticleNucleic acids research2005
Targeted 'knockdown' of spliceosome function in mammalian cells.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Nuclear mRNA maturation and mRNA export control: from trypanosomes to opisthokonts.Parasitology · 2021Review
- Disruption of exon-bridging interactions between the minor and major spliceosomes results in alternative splicing around minor introns.Nucleic acids research · 2021Article
- U1 snRNP regulates cancer cell migration and invasion in vitro.Nature communications · 2020Article
- A U2-snRNP-independent role of SF3b in promoting mRNA export.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- U1 snRNP Telescripting: Suppression of Premature Transcription Termination in Introns as a New Layer of Gene Regulation.Cold Spring Harbor perspectives in biology · 2019Review
- Trypanosomes can initiate nuclear export co-transcriptionally.Nucleic acids research · 2019Article
- Article
- Splicing inhibition decreases phosphorylation level of Ser2 in Pol II CTD.Nucleic acids research · 2015Article
- U2 snRNP is required for expression of the 3' end of genes.PloS one · 2014Article
- Nanog, Pou5f1 and SoxB1 activate zygotic gene expression during the maternal-to-zygotic transition.Nature · 2013Article
- Inhibition of mRNA maturation in trypanosomes causes the formation of novel foci at the nuclear periphery containing cytoplasmic regulators of mRNA fate.Journal of cell science · 2012Article
- Mutations in U4atac snRNA, a component of the minor spliceosome, in the developmental disorder MOPD I.Science (New York, N.Y.) · 2011Article
- Article
- Combinatorial control of signal-induced exon repression by hnRNP L and PSF.Molecular and cellular biology · 2007Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The existence of two sophisticated parallel splicing machineries in multicellular organisms has raised intriguing questions--ranging from their impact on proteome expansion to the evolution of splicing and of metazoan genomes. Exploring roles for the distinct splicing systems in vivo has, however, been restricted by the lack of techniques to selectively inhibit their function in cells. In this study, we show that morpholino oligomers complementary to the branch-site recognition elements of U2 or U12 small nuclear RNA specifically suppress the function of the two splicing systems in mammalian cells. The data provide the first evidence for a role of distinct spliceosomes in pre-mRNA splicing from endogenous mammalian genes and establish a tool to define roles for the different splicing machineries in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.