ArticleNucleic acids research2005
Modified branched peptides with a histidine-rich tail enhance in vitro gene transfection.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 79 citations in OpenAlex.
- Delivery of mRNA with Histidine-Lysine Peptides.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Receptor-Targeted Carbon Nanodot Delivery through Polymer Caging and Click Chemistry-Supported LRP1 Ligand Attachment.Polymers · 2023Article
- Marked increase in tumor transfection with a truncated branched polymer.The journal of gene medicine · 2022Article
- Amino Acids, Peptides, and Proteins: Implications for Nanotechnological Applications in Biosensing and Drug/Gene Delivery.Nanomaterials (Basel, Switzerland) · 2021Review
- Location of a single histidine within peptide carriers increases mRNA delivery.The journal of gene medicine · 2021Article
- Non-Viral Targeted Nucleic Acid Delivery: Apply Sequences for Optimization.Pharmaceutics · 2020Review
- The Multifaceted Histidine-Based Carriers for Nucleic Acid Delivery: Advances and Challenges.Pharmaceutics · 2020Review
- Peptide-Based Nanoassemblies in Gene Therapy and Diagnosis: Paving the Way for Clinical Application.Molecules (Basel, Switzerland) · 2020Review
- Synthetic Approaches for Nucleic Acid Delivery: Choosing the Right Carriers.Life (Basel, Switzerland) · 2019Review
- Solid-phase supported design of carriers for therapeutic nucleic acid delivery.Bioscience reports · 2017Review
- Functional peptides for siRNA delivery.Advanced drug delivery reviews · 2017Review
- The neuropilin-1 receptor mediates enhanced tumor delivery of H2K polyplexes.The journal of gene medicine · 2016Article
- HIV-1 drug discovery: targeting folded RNA structures with branched peptides.Organic & biomolecular chemistry · 2015Article
- Gene delivery from supercharged coiled-coil protein and cationic lipid hybrid complex.Biomaterials · 2014Article
- Enhancement of antifungal activity by integrin-targeting of branched histidine rich peptides.Journal of drug targeting · 2014Article
- Enhanced silencing and stabilization of siRNA polyplexes by histidine-mediated hydrogen bonds.Biomaterials · 2014Article
- The functions and applications of RGD in tumor therapy and tissue engineering.International journal of molecular sciences · 2013Review
- Article
- Surface-modified HK:siRNA nanoplexes with enhanced pharmacokinetics and tumor growth inhibition.Biomacromolecules · 2013Article
- Quantitative comparison between poly(L-arginine) and poly(L-lysine) at each step of polyplex-based gene transfection using a microinjection technique.Science and technology of advanced materials · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Successful gene therapy depends on the development of efficient, non-toxic gene delivery systems. To accomplish this objective, our laboratory has focused on solid-phase synthesized peptide carriers, in which the amino acid sequence can be varied precisely to augment intracellular DNA transport. We previously determined that linear and branched co-polymers of histidine and lysine in combination with liposomes enhanced the efficiency of gene transfection. In this study, we have modified two branched histidine-lysine (HK) peptides by adding a histidine-rich tail. In a variety of cell lines, this histidine-rich tail markedly improved transfection efficiency, presumably by increasing the buffering capacity of the polymer. One polymer with a histidine-rich tail, H2K4bT, compared favorably with the commonly used transfection agents. Together with modification of our transfection protocol, these improved HK peptides alone, without liposomes, are the effective carriers of plasmids into a variety of cells. We anticipate that branched HK peptides will continue to be developed as carriers of nucleic acids for in vitro and in vivo applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.