ArticleNucleic acids research2005
Selection of functional human antibodies from retroviral display libraries.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- High-throughput saturation mutagenesis generates a high-affinity antibody against SARS-CoV-2 variants using protein surface display assay on a human cell.PLoS pathogens · 2023Article
- Efficient method to optimize antibodies using avian leukosis virus display and eukaryotic cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- A Novel Dual Expression Platform for High Throughput Functional Screening of Phage Libraries in Product like Format.PloS one · 2015Article
- CARbodies: Human Antibodies Against Cell Surface Tumor Antigens Selected From Repertoires Displayed on T Cell Chimeric Antigen Receptors.Molecular therapy. Nucleic acids · 2013Article
- Library screening and receptor-directed targeting of gammaretroviral vectors.Future microbiology · 2013Review
- Human anti-EGFL7 recombinant full-length antibodies selected from a mammalian cell-based antibody display library.Molecular and cellular biochemistry · 2012Article
- RNAi-mediated gene silencing in tumour tissue using replication-competent retroviral vectors.Gene therapy · 2011Article
- Article
- The antigenome: from protein subunit vaccines to antibody treatments of bacterial infections?Advances in experimental medicine and biology · 2009Review
- Engineering higher affinity T cell receptors using a T cell display system.Journal of immunological methods · 2008Article
- Lentivirus display: stable expression of human antibodies on the surface of human cells and virus particles.PloS one · 2008Article
- Display technologies: application for the discovery of drug and gene delivery agents.Advanced drug delivery reviews · 2006Review
- Isolation of anti-CD22 Fv with high affinity by Fv display on human cells.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Selecting and screening recombinant antibody libraries.Nature biotechnology · 2005Review
- Review
- Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antibody library technology represents a powerful tool for the discovery and design of antibodies with high affinity and specificity for their targets. To extend the technique to the expression and selection of antibody libraries in an eukaryotic environment, we provide here a proof of concept that retroviruses can be engineered for the display and selection of variable single-chain fragment (scFv) libraries. A retroviral library displaying the repertoire obtained after a single round of selection of a human synthetic scFv phage display library on laminin was generated. For selection, antigen-bound virus was efficiently recovered by an overlay with cells permissive for infection. This approach allowed more than 10(3)-fold enrichment of antigen binders in a single selection cycle. After three selection cycles, several scFvs were recovered showing similar laminin-binding activities but improved expression levels in mammalian cells as compared with a laminin-specific scFv selected by the conventional phage display approach. Thus, translational problems that occur when phage-selected antibodies have to be transferred onto mammalian expression systems to exert their therapeutic potential can be avoided by the use of retroviral display libraries.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.