ArticleJournal of virology2005
3'-Terminal sequence in poliovirus negative-strand templates is the primary cis-acting element required for VPgpUpU-primed positive-strand initiation.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 46 citations in OpenAlex.
- Insight into the Life Cycle of Enterovirus-A71.Viruses · 2025Review
- Structural diversity and biological role of the 5' untranslated regions of picornavirus.RNA biology · 2023Review
- Procedures and potential pitfalls for constructing a bee-infecting RNA virus clone.Frontiers in insect science · 2022Review
- Functional advantages of triplication of the 3B coding region of the FMDV genome.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Article
- Mutational Disruption of cis-Acting Replication Element 2C in Coxsackievirus B3 Leads to 5'-Terminal Genomic Deletions.Journal of virology · 2015Article
- Initiation of protein-primed picornavirus RNA synthesis.Virus research · 2015Review
- Picornavirus RNA polyadenylation by 3D(pol), the viral RNA-dependent RNA polymerase.Virus research · 2015Review
- Human Enterovirus Nonstructural Protein 2CATPase Functions as Both an RNA Helicase and ATP-Independent RNA Chaperone.PLoS pathogens · 2015Article
- Mutational robustness and resilience of a replicative cis-element of RNA virus: Promiscuity, limitations, relevance.RNA biology · 2015Article
- Common and unique features of viral RNA-dependent polymerases.Cellular and molecular life sciences : CMLS · 2014Review
- Amiloride inhibits the initiation of Coxsackievirus and poliovirus RNA replication by inhibiting VPg uridylylation.Virology · 2014Article
- Non-template functions of viral RNA in picornavirus replication.Current opinion in virology · 2011Review
- A 5'-proximal stem-loop structure of 5' untranslated region of porcine reproductive and respiratory syndrome virus genome is key for virus replication.Virology journal · 2011Article
- An RNA element at the 5'-end of the poliovirus genome functions as a general promoter for RNA synthesis.PLoS pathogens · 2010Article
- Poly(A) at the 3' end of positive-strand RNA and VPg-linked poly(U) at the 5' end of negative-strand RNA are reciprocal templates during replication of poliovirus RNA.Journal of virology · 2010Article
- The 5'CL-PCBP RNP complex, 3' poly(A) tail and 2A(pro) are required for optimal translation of poliovirus RNA.Virology · 2010Article
- Conversion of VPg into VPgpUpUOH before and during poliovirus negative-strand RNA synthesis.Journal of virology · 2009Article
- Cis-active RNA elements (CREs) and picornavirus RNA replication.Virus research · 2009Review
- Poliovirus cis-acting replication element-dependent VPg Uridylylation lowers the Km of the initiating nucleoside triphosphate for viral RNA replication.Journal of virology · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The 5' cloverleaf in poliovirus RNA has a direct role in regulating the stability, translation, and replication of viral RNA. In this study, we investigated the role of stem a in the 5' cloverleaf in regulating the stability and replication of poliovirus RNA in HeLa S10 translation-replication reactions. Our results showed that disrupting the duplex structure of stem a destabilized viral RNA and inhibited efficient negative-strand synthesis. Surprisingly, the duplex structure of stem a was not required for positive-strand synthesis. In contrast, altering the primary sequence at the 5'-terminal end of stem a had little or no effect on negative-strand synthesis but dramatically reduced positive-strand initiation and the formation of infectious virus. The inhibition of positive-strand synthesis observed in these reactions was most likely a consequence of nucleotide alterations in the conserved sequence at the 3' ends of negative-strand RNA templates. Previous studies suggested that VPgpUpU synthesized on the cre(2C) hairpin was required for positive-strand synthesis. Therefore, these results are consistent with a model in which preformed VPgpUpU serves as the primer for positive-strand initiation on the 3'AAUUUUGUC5' sequence at the 3' ends of negative-strand templates. Our results suggest that this sequence is the primary cis-acting element that is required for efficient VPgpUpU-primed positive-strand initiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.