ArticleThe Journal of physiology2005
Visualization of localized store-operated calcium entry in mouse astrocytes. Close proximity to the endoplasmic reticulum.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
66 citing papers in PubMed, 138 citations in OpenAlex.
- Mitochondrial CaBiomolecules · 2026Review
- BTP2, a store-operated calcium channel inhibitor, attenuates morphine antinociceptive tolerance in rats.Frontiers in neuroscience · 2026Article
- Store-Operated Calcium Channels in the Nervous System.Annual review of physiology · 2025Review
- Ion channels alterations in the forebrain of high-fat diet fed rats.European journal of histochemistry : EJH · 2021Article
- Role of Oxidative Stress and CaFrontiers in cell and developmental biology · 2021Review
- Target Molecules of STIM Proteins in the Central Nervous System.Frontiers in molecular neuroscience · 2020Review
- Article
- ALS-Associated SOD1(G93A) Decreases SERCA Pump Levels and Increases Store-Operated CaInternational journal of molecular sciences · 2019Article
- Simulation of P2X-mediated calcium signalling in microglia.The Journal of physiology · 2019Article
- TRPC1 Channels Are Expressed in Pyramidal Neurons and in a Subset of Somatostatin Interneurons in the Rat Neocortex.Frontiers in neuroanatomy · 2018Article
- A superior bright NIR luminescent nanoparticle preparation and indicating calcium signaling detection in cells and small animals.Cell & bioscience · 2018Article
- Physiology of Astroglia.Physiological reviews · 2018Review
- TRPC3/6/7 Knockdown Protects the Brain from Cerebral Ischemia Injury via Astrocyte Apoptosis Inhibition and Effects on NF-кB Translocation.Molecular neurobiology · 2017Article
- TRPC1- and TRPC3-dependent CaGlia · 2017Article
- Store-operated calcium entry is essential for glial calcium signalling in CNS white matter.Brain structure & function · 2017Article
- Elevated Expression of TRPC4 in Cortical Lesions of Focal Cortical Dysplasia II and Tuberous Sclerosis Complex.Journal of molecular neuroscience : MN · 2017Article
- Orai1 and Orai3 in Combination with Stim1 Mediate the Majority of Store-operated Calcium Entry in Astrocytes.Experimental neurobiology · 2017Article
- Subcellular propagation of calcium waves in Müller glia does not require autocrine/paracrine purinergic signaling.Channels (Austin, Tex.) · 2016Article
- Beyond neurovascular coupling, role of astrocytes in the regulation of vascular tone.Neuroscience · 2016Review
- Store-Operated Calcium Entry in Müller Glia Is Controlled by Synergistic Activation of TRPC and Orai Channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Unloading of endoplasmic reticulum (ER) Ca(2+) stores activates influx of extracellular Ca(2+) through 'store-operated' Ca(2+) channels (SOCs) in the plasma membrane (PM) of most cells, including astrocytes. A key unresolved issue concerning SOC function is their spatial relationship to ER Ca(2+) stores. Here, using high resolution imaging with the membrane-associated Ca(2+) indicator, FFP-18, it is shown that store-operated Ca(2+) entry (SOCE) in primary cultured mouse cortical astrocytes occurs at plasma membrane-ER junctions. In the absence of extracellular Ca(2+), depletion of ER Ca(2+) stores using cyclopiazonic acid, an ER Ca(2+)-ATPase inhibitor, and caffeine transiently increases the sub-plasma-membrane Ca(2+) concentration ([Ca(2+)](SPM)) within a restricted space between the plasma membrane and adjacent ER. Restoration of extracellular Ca(2+) causes localized Ca(2+) influx that first increases [Ca(2+)](SPM) in the same restricted regions and then, with a delay, in ER-free regions. Antisense knockdown of the TRPC1 gene, proposed to encode endogenous SOCs, markedly reduces SOCE measured with Fura-2. High resolution immunocytochemistry with anti-TRPC1 antibody reveals that these TRPC-encoded SOCs are confined to the PM microdomains adjacent to the underlying 'junctional' ER. Thus, Ca(2+) entry through TRPC-encoded SOCs is closely linked, not only functionally, but also structurally, to the ER Ca(2+) stores.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.