ArticleInfection and immunity2005
Identification and characterization of an immunodominant 28-kilodalton Coxiella burnetii outer membrane protein specific to isolates associated with acute disease.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 51 citations in OpenAlex.
- Article
- A Comprehensive Review of the Mechanisms of Human Q Fever: Pathogenesis and Pathophysiology.Pathogens (Basel, Switzerland) · 2025Review
- Engineering Protein Nanoparticles Functionalized with an ImmunodominantBioconjugate chemistry · 2023Article
- A Novel Marine MammalPathogens (Basel, Switzerland) · 2023Article
- Clinical characteristics of acute Q fever patients in South Korea and time from symptom onset to serologic diagnosis.BMC infectious diseases · 2019Article
- Extensive genome analysis of Coxiella burnetii reveals limited evolution within genomic groups.BMC genomics · 2019Article
- Rapid and Visual Detection ofBioMed research international · 2018Article
- Interaction ofFrontiers in cellular and infection microbiology · 2017Article
- Coxiella burnetii Infects Primary Bovine Macrophages and Limits Their Host Cell Response.Infection and immunity · 2016Article
- Microevolution of the chromosomal region of acute disease antigen A (adaA) in the query (Q) fever agent Coxiella burnetii.PloS one · 2013Article
- Molecular method for the characterization of Coxiella burnetii from clinical and environmental samples: variability of genotypes in Spain.BMC microbiology · 2012Article
- Potential serodiagnostic markers for Q fever identified in Coxiella burnetii by immunoproteomic and protein microarray approaches.BMC microbiology · 2012Article
- Proteomics paves the way for Q fever diagnostics.Genome medicine · 2011Article
- A unique Coxiella burnetii lipoprotein involved in metal binding (LimB).Microbiology (Reading, England) · 2011Article
- Identification of CD4+ T cell epitopes in C. burnetii antigens targeted by antibody responses.PloS one · 2011Article
- Q Fever: current state of knowledge and perspectives of research of a neglected zoonosis.International journal of microbiology · 2011Article
- Immunization with truncated recombinant protein SpaC of Erysipelothrix rhusiopathiae strain 715 serovar 18 confers protective immunity against challenge with various serovars.Clinical and vaccine immunology : CVI · 2010Article
- Characterization of a Coxiella burnetii ftsZ mutant generated by Himar1 transposon mutagenesis.Journal of bacteriology · 2009Article
- Genetic and antigenic diversity of the surface protective antigen proteins of Erysipelothrix rhusiopathiae.Clinical and vaccine immunology : CVI · 2007Article
- Proteome and antigen profiling of Coxiella burnetii developmental forms.Infection and immunity · 2007Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Coxiella burnetii causes acute Q fever in humans and occasional chronic infections that typically manifest as endocarditis or hepatitis. Isolates associated with acute disease were found to be distinct from a group of chronic disease isolates by a variety of biochemical parameters and in a guinea pig fever model of acute disease, suggesting a difference in virulence potential. We compared antigenic polypeptides among C. burnetii isolates and found an immunodominant 28-kDa protein in acute group isolates but not in chronic group isolates (T. Ho, A. Hotta, G. Q. Zhang, S. V. Nguyen, M. Ogawa, T. Yamaguchi, H. Fukushi, and K. Hirai, Microbiol. Immunol. 42:81-85, 1998). In order to clone the adaA gene, the N-terminal amino acid sequence of adaA was determined and a 59-bp fragment was amplified from Nine Mile phase I DNA by PCR. The putative gene fragment was used to screen a lambda ZAP II genomic DNA library, and an open reading frame expressing a 28-kDa immunoreactive protein was identified. Sequence analysis predicted a gene encoding an approximately 28-kDa mature protein with a typical signal sequence. The adaA (acute disease antigen A) gene was detected in acute group C. burnetii isolates but not identified in chronic group isolates by PCR and Southern blotting. A typical signal peptide was predicted in adaA, and specific antibody to adaA reacted with the purified membrane fraction of acute group isolates by Western blotting, suggesting that adaA is exposed on the outer surface of C. burnetii. adaA was overexpressed in pET23a as a fusion protein in Escherichia coli to develop anti-recombinant adaA (anti-radaA) specific antibody, which recognized a approximately 28-kDa band in acute group isolates but not in chronic group isolates. In addition, immunoblotting indicates that radaA reacted with sera derived from animals infected with acute group isolates but did not react with sera from animals infected with chronic group isolates. These results support the idea that an adaA gene-targeted PCR assay and an radaA antigen-based serodiagnostic test may be useful for differential diagnosis of acute and chronic Q fever.
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