ArticleInfection and immunity2005
Translocation and surface expression of lipidated serogroup B capsular Polysaccharide in Neisseria meningitidis.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 64 citations in OpenAlex.
- The unique Legionella longbeachae capsule favors intracellular replication and immune evasion.PLoS pathogens · 2024Article
- Pathogenic determinants ofFrontiers in pediatrics · 2022Review
- Article
- The sweet side of the pathogenic Neisseria: the role of glycan interactions in colonisation and disease.Pathogens and disease · 2017Review
- The primary transcriptome of Neisseria meningitidis and its interaction with the RNA chaperone Hfq.Nucleic acids research · 2017Article
- Regulation of capsule in Neisseria meningitidis.Critical reviews in microbiology · 2016Review
- Genomic Investigation Reveals Highly Conserved, Mosaic, Recombination Events Associated with Capsular Switching among Invasive Neisseria meningitidis Serogroup W Sequence Type (ST)-11 Strains.Genome biology and evolution · 2016Article
- Genetic and Molecular Basis of Kingella kingae Encapsulation.Infection and immunity · 2016Article
- Masquerading microbial pathogens: capsular polysaccharides mimic host-tissue molecules.FEMS microbiology reviews · 2014Review
- KpsC and KpsS are retaining 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) transferases involved in synthesis of bacterial capsules.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Conserved glycolipid termini in capsular polysaccharides synthesized by ATP-binding cassette transporter-dependent pathways in Gram-negative pathogens.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Description and nomenclature of Neisseria meningitidis capsule locus.Emerging infectious diseases · 2013Article
- Attachment and invasion of Neisseria meningitidis to host cells is related to surface hydrophobicity, bacterial cell size and capsule.PloS one · 2013Article
- Article
- Development of a multiplex PCR assay for detection and genogrouping of Neisseria meningitidis.Journal of clinical microbiology · 2012Article
- Neisseria meningitidis capsular polysaccharides induce inflammatory responses via TLR2 and TLR4-MD-2.Journal of leukocyte biology · 2011Article
- Article
- ABC transporters involved in export of cell surface glycoconjugates.Microbiology and molecular biology reviews : MMBR · 2010Review
- Requirement of NMB0065 for connecting assembly and export of sialic acid capsular polysaccharides in Neisseria meningitidis.Microbes and infection · 2010Article
- Biology and pathogenesis of the evolutionarily successful, obligate human bacterium Neisseria meningitidis.Vaccine · 2009Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The capsule of N. meningitidis serogroup B, (alpha2-->8)-linked polysialic acid and the capsules of other meningococcal serogroups and of other gram-negative bacterial pathogens are anchored in the outer membrane through a 1,2-diacylglycerol moiety. Previous work on the meningococcal cps complex in Escherichia coli K-12 indicated that deletion of genes designated lipA and lipB caused intracellular accumulation of hyperelongated capsule polymers lacking the phospholipid substitution. To better understand the role of lip and lipB in capsule expression in a meningococcal background, the location, sequence, and relationship to related bacterial capsule genes were defined and specific mutations in lipA and lipB were generated in the serogroup B meningococcal strain NMB. The lipA and lipB genes are located on the 3' end of the ctr operon and are most likely transcribed independently. Inactivation of lipA, lipB, and both resulted in the same total levels of capsular polymer production as in the parental controls; however, these mutants were as sensitive as an unencapsulated mutant to killing by normal human serum. Immunogold electron microscopy and flow cytometric analyses revealed intracellular inclusions of capsular polymers in lipA, lipB, and lipA lipB mutants. Capsular polymers purified from lipA, lipB, and lipA lipB mutants were lipidated. The phospholipid anchor was shown by gas chromatography-mass spectroscopy analysis to be a phosphodiester-linked 1,2-dipalmitoyl (C16:0) glycerol moiety and was identical in structure to that found on the wild-type meningococcal capsule polymers. Thus, lipA and lipB do not encode proteins responsible for diacylglycerophosphatidic acid substitution of the meningococcal capsule polymer; rather, they are required for proper translocation and surface expression of the lipidated polymer.
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