ArticleInfection and immunity2005
Recombinant guinea pig tumor necrosis factor alpha stimulates the expression of interleukin-12 and the inhibition of Mycobacterium tuberculosis growth in macrophages.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 53 citations in OpenAlex.
- Molecular drivers of non-alcoholic steatohepatitis are sustained in mild-to-late fibrosis progression in a guinea pig model.Molecular genetics and genomics : MGG · 2019Article
- Mycobacterium tuberculosis sigE mutant ST28 used as a vaccine induces protective immunity in the guinea pig model.Tuberculosis (Edinburgh, Scotland) · 2017Article
- Molecular and biochemical characterization of recombinant guinea pig tumor necrosis factor-alpha.Mediators of inflammation · 2015Article
- Latent tuberculosis infection: myths, models, and molecular mechanisms.Microbiology and molecular biology reviews : MMBR · 2014Review
- Immune subversion by Mycobacterium tuberculosis through CCR5 mediated signaling: involvement of IL-10.PloS one · 2014Article
- Superoxide dismutase 2 as a marker to differentiate tuberculous pleural effusions from malignant pleural effusions.Clinics (Sao Paulo, Brazil) · 2014Article
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- Kinetics of IFN-gamma and TNF-alpha gene expression and their relationship with disease progression after infection with Mycobacterium tuberculosis in guinea pigs.Yonsei medical journal · 2013Article
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- The in vivo immunomodulatory effect of recombinant tumour necrosis factor-alpha in guinea pigs vaccinated with Mycobacterium bovis bacille Calmette-Guérin.Clinical and experimental immunology · 2011Article
- The stringent response is required for full virulence of Mycobacterium tuberculosis in guinea pigs.The Journal of infectious diseases · 2010Article
- Guinea pig neutrophil-macrophage interactions during infection with Mycobacterium tuberculosis.Microbes and infection · 2010Article
- Evaluation of standard chemotherapy in the guinea pig model of tuberculosis.Antimicrobial agents and chemotherapy · 2010Article
- Kinetics of the immune response profile in guinea pigs after vaccination with Mycobacterium bovis BCG and infection with Mycobacterium tuberculosis.Infection and immunity · 2009Article
- Increased risk of tuberculosis in patients treated with antitumor necrosis factor alpha.Clinical rheumatology · 2009Article
- Dietary polyunsaturated fatty acids modulate resistance to Mycobacterium tuberculosis in guinea pigs.The Journal of nutrition · 2008Article
- The guinea pig as a model of infectious diseases.Comparative medicine · 2008Review
- Cytokine profiles in primary and secondary pulmonary granulomas of Guinea pigs with tuberculosis.American journal of respiratory cell and molecular biology · 2008Article
- Lung macrophages from bacille Calmette-Guérin-vaccinated guinea pigs suppress T cell proliferation but restrict intracellular growth of M. tuberculosis after recombinant guinea pig interferon-gamma activation.Clinical and experimental immunology · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Tumor necrosis factor alpha (TNF-alpha) plays an important role in the host immune response to infection with the intracellular pathogen Mycobacterium tuberculosis. It is essential for the formation of protective tuberculous granulomas and regulates the expression of other cytokines which contribute to a protective immune response. Interleukin-12 (IL-12) is known to promote a Th1 response, which is essential for antimycobacterial resistance. Recombinant guinea pig TNF-alpha (rgpTNF-alpha) protein (17 kDa) was purified, and its bioactivity was confirmed by its cytotoxicity for L929 fibroblasts. High titers of polyclonal anti-gpTNF-alpha antibody were obtained by immunization of rabbits. Resident alveolar and peritoneal macrophages were isolated from guinea pigs and infected with either the H37Ra or H37Rv strain of M. tuberculosis. The mRNA levels for TNF-alpha and IL-12 p40 were measured using real-time PCR. IL-12 p40 mRNA was up-regulated in a dose-dependent manner by rgpTNF-alpha alone. In infected macrophages, a lower dose of rgpTNF-alpha intensified the mRNA levels of TNF-alpha and IL-12 p40. However, higher doses of rgpTNF-alpha suppressed TNF-alpha and IL-12 p40 mRNA. The antimycobacterial activity of macrophages was assessed by metabolic labeling of M. tuberculosis with [3H]uracil. Resident alveolar and peritoneal macrophages treated with anti-gpTNF-alpha antibody to block endogenous TNF-alpha exhibited increased intracellular mycobacterial growth. These data suggest that the dose of TNF-alpha is crucial to the stimulation of optimal expression of protective cytokines and that TNF-alpha contributes to the control of mycobacterial replication to promote host resistance against M. tuberculosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.