ArticleInfection and immunity2005
The StcE protease contributes to intimate adherence of enterohemorrhagic Escherichia coli O157:H7 to host cells.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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76 citing papers in PubMed, 132 citations in OpenAlex.
- Enzyme-mediated remodeling of the extracellular matrix and glycocalyx to enhance immunotherapy in solid tumors.Bioengineering & translational medicine · 2026Review
- Comprehensive Genomic Analysis of the pO157 Plasmid in Enterohemorrhagic Escherichia coli O157:H7 Reveals Non-synonymous Variation in Virulence Factors.Current microbiology · 2026Article
- Review
- Bacterial zinc metalloproteases as facilitators of pathogenic success: a review.Archives of microbiology · 2026Review
- Mucinase fromFrontiers in microbiology · 2026Article
- CanineMicrobial genomics · 2026Article
- Bacterial warfare is associated with virulence and antimicrobial resistance.Nature communications · 2025Article
- Microbial binding module employs sophisticated clustered saccharide patches to selectively adhere to mucins.Nature communications · 2025Article
- Vat-Mediated Mucus Penetration Enables Genotoxic Activity ofInternational journal of molecular sciences · 2025Article
- Mucins and Their Roles in Asthma.Immunological reviews · 2025Review
- Stepwise assembly and release of Tc toxins from Yersinia entomophaga.Nature microbiology · 2024Article
- Structural and mechanistic insights into the cleavage of clustered O-glycan patches-containing glycoproteins by mucinases of the human gut.Nature communications · 2022Article
- Article
- Mucus and Mucins: The Underappreciated Host Defence System.Frontiers in cellular and infection microbiology · 2022Review
- Independent Component Analysis Identifies the Modulons Expanding the Transcriptional Regulatory Networks of EnterohemorrhagicFrontiers in microbiology · 2022Article
- Phage-encoded carbohydrate-interacting proteins in the human gut.Frontiers in microbiology · 2022Review
- Comparative genomics of Chinese and international isolates ofMicrobial genomics · 2021Article
- Pangenome analysis and virulence profiling of Streptococcus intermedius.BMC genomics · 2021Article
- Display of the human mucinome with defined O-glycans by gene engineered cells.Nature communications · 2021Article
- Article
16 more citing papers are in PubMed but not listed here.
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4 authors at 1 institution in 1 country.
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Abstract
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a diarrheal pathogen that causes attaching and effacing (A/E) lesions on intestinal epithelial cells. Strains of the O157 serogroup carry the large virulence plasmid pO157, which encodes the etp type II secretion system that secretes the genetically linked zinc metalloprotease StcE. The Ler regulator controls expression of many genes involved in A/E lesion formation, as well as StcE, suggesting StcE may be important at a similar time during colonization. Our laboratory has previously demonstrated that StcE cleaves C1-esterase inhibitor, a regulator of multiple inflammation pathways. Here we report two new substrates for StcE, mucin 7 and glycoprotein 340, and that purified StcE reduces the viscosity of human saliva. We tested the hypothesis that StcE contributes to intimate adherence of EHEC to host cells by cleavage of glycoproteins from the cell surface. The fluorescent actin stain (FAS) test was used to observe the intimate adherence represented by fluorescently stained bacteria colocalized with regions of bundled actin formed on HEp-2 cells. An E. coli O157:H7 strain with a stcE gene deletion was not affected in its ability to generally adhere to HEp-2 cells, but it did score threefold lower on the FAS test than wild-type or complemented strains. Addition of exogenous recombinant StcE increased intimate adherence of the mutant to wild-type levels. Thus, StcE may help block host clearance of E. coli O157:H7 by destruction of some classes of glycoproteins, and it contributes to intimate adherence of E. coli O157:H7 to the HEp-2 cell surface.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.