ArticleVeterinary research communications2005
Expression of mRNA encoding interleukin (IL)-10, IL-12p35 and IL-12p40 in lungs from pigs experimentally infected with Actinobacillus pleuropneumoniae.
Article in Veterinary research communications, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 18 citations in OpenAlex.
- Candidate genes and gene markers for the resistance to porcine pleuropneumonia.Mammalian genome : official journal of the International Mammalian Genome Society · 2020Article
- Frequency of Th17 cells correlates with the presence of lung lesions in pigs chronically infected with Actinobacillus pleuropneumoniae.Veterinary research · 2017Article
- Pathway deregulation and expression QTLs in response to Actinobacillus pleuropneumoniae infection in swine.Mammalian genome : official journal of the International Mammalian Genome Society · 2014Article
- Identification of QTL affecting resistance/susceptibility to acute Actinobacillus pleuropneumoniae infection in swine.Mammalian genome : official journal of the International Mammalian Genome Society · 2014Article
- Transcriptional profiling of hilar nodes from pigs after experimental infection with Actinobacillus pleuropneumoniae.International journal of molecular sciences · 2013Article
- Transcriptional profiling of swine lung tissue after experimental infection with Actinobacillus pleuropneumoniae.International journal of molecular sciences · 2013Article
- Molecular characterisation of the early response in pigs to experimental infection with Actinobacillus pleuropneumoniae using cDNA microarrays.Acta veterinaria Scandinavica · 2007Article
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5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expression of mRNA encoding interleukin (IL)-10, IL-12p35 and IL-12p40 was studied, by reverse transcription-polymerase chain reaction and by in situ hybridization with a non-radioactive digoxigenin-labelled cDNA probe, in formalin-fixed, paraffin-wax-embedded lung tissue from pigs experimentally infected with Actinobacillus pleuropneumoniae. Forty-eight 7-week-old colostrum-deprived pigs were randomly allocated to infected (n = 24) or control (n = 24) groups. Three pigs from each group were euthanized at 3, 6, 9, 12, 24, 36, 48 and 60 h post inoculation (hpi). IL-10 mRNA was detected in the lung at 3 hpi, numbers of cells positive for IL-10 increasing at 36 hpi. IL-12p35 mRNA was detected in the lung at 3 hpi, numbers of cells positive for IL-12p35 increasing at 36 and 48 hpi and rapidly decreasing thereafter whereas IL-12p40 mRNA was constitutively expressed at low levels during the experiment. Hybridization signals for IL-10, IL-12p35 and IL-12p40 were always associated with inflammation, in particular with macrophages and neutrophils within alveolar spaces. Expression of these cytokines was minimal in non-lesional lung of A. pleuropneumoniae-infected pigs and in normal lung from control pigs. In situ hybridization of A. pleuropneumoniae and these cytokines in serial sections of lung tissues indicated close co-localization of A. pleuropneumoniae and these cytokines in pleuropneumonia. The results suggest that the expression of IL-10 and IL-12 play a role in pathogenesis of A. pleuropneumoniae infection.
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