ArticleAntimicrobial agents and chemotherapy2005
Global expression of prophage genes in Escherichia coli O157:H7 strain EDL933 in response to norfloxacin.
Article in Antimicrobial agents and chemotherapy, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 84 citations in OpenAlex.
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- Fighting Fire with Fire: Phage Potential for the Treatment ofAntibiotics (Basel, Switzerland) · 2018Article
- Recombinant biosynthesis of bacterial cellulose in genetically modified Escherichia coli.Bioprocess and biosystems engineering · 2018Article
- Pch Genes Control Biofilm and Cell Adhesion in a Clinical Serotype O157:H7 Isolate.Frontiers in microbiology · 2018Article
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- Sulfamethoxazole - Trimethoprim represses csgD but maintains virulence genes at 30°C in a clinical Escherichia coli O157:H7 isolate.PloS one · 2018Article
- Induction of Shiga Toxin-Encoding Prophage by Abiotic Environmental Stress in Food.Applied and environmental microbiology · 2017Article
- Global gene expression inGut pathogens · 2017Article
- Escherichia coli O157:H7 Strain EDL933 Harbors Multiple Functional Prophage-Associated Genes Necessary for the Utilization of 5-N-Acetyl-9-O-Acetyl Neuraminic Acid as a Growth Substrate.Applied and environmental microbiology · 2016Article
- Expansion of Shiga Toxin-Producing Escherichia coli by Use of Bovine Antibiotic Growth Promoters.Emerging infectious diseases · 2016Article
- Article
- Microbial forensics: predicting phenotypic characteristics and environmental conditions from large-scale gene expression profiles.PLoS computational biology · 2015Article
- The gut bacterium Bacteroides thetaiotaomicron influences the virulence potential of the enterohemorrhagic Escherichia coli O103:H25.PloS one · 2015Article
- Microbiological effects of sublethal levels of antibiotics.Nature reviews. Microbiology · 2014Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the influence of a low concentration of the gyrase inhibitor norfloxacin on the transcriptome of enterohemorrhagic Escherichia coli O157:H7 strain EDL933. For this purpose, we used a commercial DNA microarray containing oligonucleotides specific for E. coli O157:H7 strains EDL933 and RIMD0509952 and E. coli K-12 strain MG1655. Under the conditions applied, 5,963 spots (94% of all spots) could be analyzed. Among these, 118 spots (P < 0.05) indicated transcriptional upregulation and 122 spots (P < 0.05) indicated transcriptional downregulation of the E. coli genes present on the array. Eighty-five upregulated EDL933 genes were phage borne. Fifty-two of them could be ascribed to the Shiga toxin-encoding phages (Stx phages) BP-933W and CP-933V; the other 33 genes belonged to non-Stx prophage elements in the EDL933 genome. Genes present in the BP-933W prophage genome were induced most strongly up to 158-fold in the case of stxA(2) upon induction with norfloxacin. Twenty-two additional upregulated genes appeared to be E. coli O157:H7 strain RIMD0509952-specific phage elements, and the remaining 11 genes were related mainly to recombination and stress functions. Downregulation was indicated predominantly for genes responsible for bacterial primary metabolism, such as energy production, cell division, and amino acid biosynthesis. Interestingly, some genes present in the locus of enterocyte effacement appeared to be downregulated. The results of the study have shown that a low concentration of norfloxacin has profound effects on the transcriptome of E. coli O157:H7.
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