ArticleProceedings of the National Academy of Sciences of the United States of America2005
HLA-restricted epitope identification and detection of functional T cell responses by using MHC-peptide and costimulatory microarrays.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 76 citations in OpenAlex.
- Marked differences in human melanoma antigen-specific T cell responsiveness after vaccination using a functional microarray.PLoS medicine · 2005Trial
- T Cell Assay to Track Presentation of an Influenza Hemagglutinin Epitope Presented by the MHC-II Protein HLA-DR1.Methods in molecular biology (Clifton, N.J.) · 2026Article
- State of the art in epitope mapping and opportunities in COVID-19.Future science OA · 2023Review
- Defining antigen targets to dissect vaccinia virus and monkeypox virus-specific T cell responses in humans.Cell host & microbe · 2022Article
- Development of Peptide Vaccines in Dengue.Current pharmaceutical design · 2018Review
- Frequency and reactivity of antigen-specific T cells were concurrently measured through the combination of artificial antigen-presenting cell, MACS and ELISPOT.Scientific reports · 2017Article
- Evolution of MHC-based technologies used for detection of antigen-responsive T cells.Cancer immunology, immunotherapy : CII · 2017Review
- A Simple Microfluidic Platform for Long-Term Analysis and Continuous Dual-Imaging Detection of T-Cell Secreted IFN-γ and IL-2 on Antibody-Based Biochip.Biosensors · 2015Article
- Drug-eluting microarrays to identify effective chemotherapeutic combinations targeting patient-derived cancer stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Identification and HLA-tetramer-validation of human CD4+ and CD8+ T cell responses against HCMV proteins IE1 and IE2.PloS one · 2014Article
- Development of a hypersensitive periodate-cleavable amino acid that is methionine- and disulfide-compatible and its application in MHC exchange reagents for T cell characterisation.Chembiochem : a European journal of chemical biology · 2013Article
- Life on a microarray: assessing live cell functions in a microarray format.Cellular and molecular life sciences : CMLS · 2012Review
- Missing: a diagnostic technique to enumerate antigen-specific T cells.Critical reviews in oncology/hematology · 2012Review
- Antigen-specific T cell phenotyping microarrays using grating coupled surface plasmon resonance imaging and surface plasmon coupled emission.Biosensors & bioelectronics · 2012Article
- Novel cellular microarray assay for profiling T-cell peptide antigen specificities.Journal of proteome research · 2010Article
- Peptide microarrays for the profiling of cytotoxic T-lymphocyte activity using minimum numbers of cells.Cancer immunology, immunotherapy : CII · 2010Article
- MHC-based detection of antigen-specific CD8+ T cell responses.Cancer immunology, immunotherapy : CII · 2010Review
- Peptide-MHC cellular microarray with innovative data analysis system for simultaneously detecting multiple CD4 T-cell responses.PloS one · 2010Article
- Advances in the design and delivery of peptide subunit vaccines with a focus on toll-like receptor agonists.Expert review of vaccines · 2010Review
- High throughput T epitope mapping and vaccine development.Journal of biomedicine & biotechnology · 2010Review
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Identification of T cell epitopes is a vital but often slow and difficult step in studying the immune response to infectious agents and autoantigens. We report a spatially addressable technique for screening large numbers of T cell epitopes for both specific antigen recognition and functional activity induced. This system uses microarrays of immobilized, recombinant MHC-peptide complexes, costimulatory molecules, and cytokine-capture antibodies. The array elements act as synthetic antigen-presenting cells and specifically elicit T cell responses, including adhesion, secretion of cytokines, and modulation of surface markers. The method allows facile identification of pertinent T cell epitopes in a large number of candidates and simultaneous determination of the functional outcome of the interaction. Using this method, we have characterized the activation of human CD4(+) and CD8(+) T cells responding to vaccinia, influenza, HIV-1, and Epstein-Barr viruses.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.