ArticleMolecular biology of the cell2005
A Dictyostelium homologue of WASP is required for polarized F-actin assembly during chemotaxis.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 84 citations in OpenAlex.
- Ancestral TALE homeobox protein transcription factor regulates actin dynamics and cellular activities of protozoan parasite Entamoeba invadens.Molecular microbiology · 2024Article
- Why Does Synergistic Activation of WASP, but Not N-WASP, by Cdc42 and PIPJournal of molecular biology · 2023Article
- Cdc42/Rac Interactive Binding Containing Effector Proteins in Unicellular Protozoans With Reference to Human Host: Locks of the Rho Signaling.Frontiers in genetics · 2022Review
- Review
- Ras, PI3K and mTORC2 - three's a crowd?Journal of cell science · 2020Review
- WasC, a WASP family protein, is involved in cell adhesion and migration through regulation of F-actin polymerization in Dictyostelium.Journal of microbiology (Seoul, Korea) · 2020Article
- An endogenous chemorepellent directs cell movement by inhibiting pseudopods at one side of cells.Molecular biology of the cell · 2019Article
- WASP family proteins and formins compete in pseudopod- and bleb-based migration.The Journal of cell biology · 2018Article
- WASP and SCAR are evolutionarily conserved in actin-filled pseudopod-based motility.The Journal of cell biology · 2017Article
- Reliable handling of highly A/T-rich genomic DNA for efficient generation of knockin strains of Dictyostelium discoideum.BMC biotechnology · 2016Article
- Coronin7 regulates WASP and SCAR through CRIB mediated interaction with Rac proteins.Scientific reports · 2015Article
- Absence of catalytic domain in a putative protein kinase C (PkcA) suppresses tip dominance in Dictyostelium discoideum.Developmental biology · 2015Article
- Moving towards a paradigm: common mechanisms of chemotactic signaling in Dictyostelium and mammalian leukocytes.Cellular and molecular life sciences : CMLS · 2014Review
- PakD, a putative p21-activated protein kinase in Dictyostelium discoideum, regulates actin.Eukaryotic cell · 2014Article
- An attenuating role of a WASP-related protein, WASP-B, in the regulation of F-actin polymerization and pseudopod formation via the regulation of RacC during Dictyostelium chemotaxis.Biochemical and biophysical research communications · 2013Article
- Dictyostelium discoideum SecG interprets cAMP-mediated chemotactic signals to influence actin organization.Cytoskeleton (Hoboken, N.J.) · 2013Article
- Article
- SCAR knockouts in Dictyostelium: WASP assumes SCAR's position and upstream regulators in pseudopods.The Journal of cell biology · 2012Article
- Impact of the carbazole derivative wiskostatin on mechanical stability and dynamics of motile cells.Journal of muscle research and cell motility · 2012Article
- Molecular basis of dynamic relocalization of Dictyostelium myosin IB.The Journal of biological chemistry · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The actin cytoskeleton controls the overall structure of cells and is highly polarized in chemotaxing cells, with F-actin assembled predominantly in the anterior leading edge and to a lesser degree in the cell's posterior. Wiskott-Aldrich syndrome protein (WASP) has emerged as a central player in controlling actin polymerization. We have investigated WASP function and its regulation in chemotaxing Dictyostelium cells and demonstrated the specific and essential role of WASP in organizing polarized F-actin assembly in chemotaxing cells. Cells expressing very low levels of WASP show reduced F-actin levels and significant defects in polarized F-actin assembly, resulting in an inability to establish axial polarity during chemotaxis. GFP-WASP preferentially localizes at the leading edge and uropod of chemotaxing cells and the B domain of WASP is required for the localization of WASP. We demonstrated that the B domain binds to PI(4,5)P2 and PI(3,4,5)P3 with similar affinities. The interaction between the B domain and PI(3,4,5)P3 plays an important role for the localization of WASP to the leading edge in chemotaxing cells. Our results suggest that the spatial and temporal control of WASP localization and activation is essential for the regulation of directional motility.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.