ArticleCellular and molecular life sciences : CMLS2005
Confocal microscopy and biochemical analysis reveal spatial and functional separation between anandamide uptake and hydrolysis in human keratinocytes.
Article in Cellular and molecular life sciences : CMLS, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 61 citations in OpenAlex.
- Endocannabinoids at the synapse and beyond: implications for neuropsychiatric disease pathophysiology and treatment.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2023Review
- Mechanisms of endocannabinoid transport in the brain.British journal of pharmacology · 2022Review
- Effects of Rare Phytocannabinoids on the Endocannabinoid System of Human Keratinocytes.International journal of molecular sciences · 2022Article
- Cannabinoid Signaling in the Skin: Therapeutic Potential of the "C(ut)annabinoid" System.Molecules (Basel, Switzerland) · 2019Review
- Epigenetic control of skin differentiation genes by phytocannabinoids.British journal of pharmacology · 2013Article
- Increased anandamide uptake by sensory neurons contributes to hyperalgesia in a model of cancer pain.Neurobiology of disease · 2013Article
- Fatty acid-binding proteins transport N-acylethanolamines to nuclear receptors and are targets of endocannabinoid transport inhibitors.The Journal of biological chemistry · 2012Article
- Pitfalls and solutions in assaying anandamide transport in cells.Journal of lipid research · 2010Article
- Lipid droplets are novel sites of N-acylethanolamine inactivation by fatty acid amide hydrolase-2.The Journal of biological chemistry · 2010Article
- Identification of intracellular carriers for the endocannabinoid anandamide.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Aspects of endocannabinoid signaling in periimplantation biology.Molecular and cellular endocrinology · 2008Review
- Inhibition of the cellular uptake of anandamide by genistein and its analogue daidzein in cells with different levels of fatty acid amide hydrolase-driven uptake.British journal of pharmacology · 2007Article
- Endocannabinoid liberation from neurons in transsynaptic signaling.Journal of molecular neuroscience : MN · 2007Review
- The 'specific' tyrosine kinase inhibitor genistein inhibits the enzymic hydrolysis of anandamide: implications for anandamide uptake.British journal of pharmacology · 2007Article
- Is there a temperature-dependent uptake of anandamide into cells?British journal of pharmacology · 2006Article
- New potent and selective inhibitors of anandamide reuptake with antispastic activity in a mouse model of multiple sclerosis.British journal of pharmacology · 2006Article
- Identification of a high-affinity binding site involved in the transport of endocannabinoids.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Cannabinol modulates the endocannabinoid system and shows TRPV1-mediated anti-inflammatory properties in human keratinocytes.BioFactors (Oxford, England)Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The signaling activity of anandamide (AEA) is terminated by its uptake across the cellular membrane and subsequent intracellular hydrolysis by the fatty acid amide hydrolase (FAAH). To date, the existence of an AEA membrane transporter (AMT) independent of FAAH activity remains questionable, although it has been recently corroborated by pharmacological and genetic data. We performed confocal microscopy and biochemical analysis in human HaCaT keratinocytes, in order to study the cellular distribution of AMT and FAAH. We found that FAAH is intracellularly localized as a punctate staining partially overlapping with the endoplasmic reticulum. Consistently, subcellular fractionation and reconstitution of vesicles from membranes of different compartments demonstrated that FAAH activity was localized mainly in microsomal fractions, whereas AMT activity was almost exclusively in plasma membranes. These results provide the first morphological and biochemical evidence to support the view that transport and hydrolysis are two spatially and functionally distinct processes in AEA degradation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.