ArticleEMBO reports2005
The SNARE Ykt6 is released from yeast vacuoles during an early stage of fusion.
Article in EMBO reports, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Homozygous missense variants in YKT6 result in loss of function and are associated with developmental delay, with or without severe infantile liver disease and risk for hepatocellular carcinoma.Genetics in medicine : official journal of the American College of Medical Genetics · 2024Article
- Cell biology of protein-lipid conjugation.Cell structure and function · 2023Review
- ULK1-mediated phosphorylation regulates the conserved role of YKT6 in autophagy.Journal of cell science · 2023Article
- Function of the SNARE Ykt6 on autophagosomes requires the Dsl1 complex and the Atg1 kinase complex.EMBO reports · 2020Article
- Phosphorylation of Ykt6 SNARE Domain Regulates Its Membrane Recruitment and Activity.Biomolecules · 2020Article
- Non-canonical role of the SNARE protein Ykt6 in autophagosome-lysosome fusion.PLoS genetics · 2018Article
- Phylogenetic Analysis of the vesicular fusion SNARE machinery revealing its functional divergence across Eukaryotes.Bioinformation · 2018Article
- Steric hindrance of SNARE transmembrane domain organization impairs the hemifusion-to-fusion transition.EMBO reports · 2016Article
- Distinct palmitoylation events at the amino-terminal conserved cysteines of Env7 direct its stability, localization, and vacuolar fusion regulation in S. cerevisiae.The Journal of biological chemistry · 2014Article
- Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole.Molecular and cellular biology · 2013Article
- A lipid-anchored SNARE supports membrane fusion.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Evidence for prenylation-dependent targeting of a Ykt6 SNARE in Plasmodium falciparum.Molecular and biochemical parasitology · 2011Article
- Sec18p and Vam7p remodel trans-SNARE complexes to permit a lipid-anchored R-SNARE to support yeast vacuole fusion.The EMBO journal · 2007Article
- Unique self-palmitoylation activity of the transport protein particle component Bet3: a mechanism required for protein stability.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Article
- Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p.The EMBO journal · 2006Article
- The multi-functional SNARE protein Ykt6 in autophagosomal fusion processes.Cell cycle (Georgetown, Tex.)Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The farnesylated SNARE (N-ethylmaleimide-sensitive factor attachment protein receptor) Ykt6 mediates protein palmitoylation at the yeast vacuole by means of its amino-terminal longin domain. Ykt6 is localized equally to membranes and the cytosol, although it is unclear how this distribution is mediated. We now show that Ykt6 is released efficiently from vacuoles during an early stage of yeast vacuole fusion. This release is dependent on the disassembly of vacuolar SNAREs (priming). In recent literature, it had been demonstrated for mammalian Ykt6 that the membrane-bound form is both palmitoylated and farnesylated at its carboxy-terminal CAAX box, whereas soluble Ykt6 is only farnesylated. In agreement with this, we find that yeast Ykt6 becomes palmitoylated in vitro at its C-terminal CAAX motif. Mutagenesis of the potential palmitoylation site in yeast Ykt6 prevents stable membrane association and is lethal. On the basis of these and other findings, we speculate that Ykt6 is released from membranes by depalmitoylation. Such a mechanism could enable recycling of this lipid-anchored SNARE from the vacuole independent of retrograde transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.