ArticleBiomaterials2005
A system for the direct co-culture of endothelium on smooth muscle cells.
Article in Biomaterials, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 80 citations in OpenAlex.
- Glutathione peroxidase mimic-engineered α-lactalbumin self-assembling coatings tailor immunomodulatory vascular stents for suppressing restenosis.Bioactive materials · 2026Article
- Migration of co-cultured endothelial and smooth muscle cells on material surfaces.Regenerative biomaterials · 2026Article
- Innovative Atherosclerosis Models: Advancing Pathophysiology and Translational Research.Research (Washington, D.C.) · 2025Review
- A Co-Culture System for Studying Cellular Interactions in Vascular Disease.Bioengineering (Basel, Switzerland) · 2024Review
- A Review of Functional Analysis of Endothelial Cells in Flow Chambers.Journal of functional biomaterials · 2022Review
- Lymphangion-chip: a microphysiological system which supports co-culture and bidirectional signaling of lymphatic endothelial and muscle cells.Lab on a chip · 2021Article
- Durable endothelium-mimicking coating for surface bioengineering cardiovascular stents.Bioactive materials · 2021Article
- Article
- Design considerations for engineering 3D models to study vascular pathologies in vitro.Acta biomaterialia · 2021Review
- A high-throughput microfluidic bilayer co-culture platform to study endothelial-pericyte interactions.Scientific reports · 2021Article
- Multiphoton-Guided Creation of Complex Organ-Specific Microvasculature.Advanced healthcare materials · 2021Article
- Construction of tissue engineering bone with the co‑culture system of ADSCs and VECs on partially deproteinized biologic boneMolecular medicine reports · 2021Article
- Endothelium-Mimicking Multifunctional Coating Modified Cardiovascular Stents via a Stepwise Metal-Catechol-(Amine) Surface Engineering Strategy.Research (Washington, D.C.) · 2020Article
- Orthogonal co-cultivation of smooth muscle cell and endothelial cell layers to constructBiomicrofluidics · 2019Article
- Optimizing endothelial cell functionalization for cell therapy of vascular proliferative disease using a direct contact co-culture system.Drug delivery and translational research · 2018Article
- A biomimetic microfluidic model to study signalling between endothelial and vascular smooth muscle cells under hemodynamic conditions.Lab on a chip · 2018Article
- Cellular Self-Assembly with Microsphere Incorporation for Growth Factor Delivery Within Engineered Vascular Tissue Rings.Tissue engineering. Part A · 2017Article
- Hydraulic Conductivity of Smooth Muscle Cell-Initiated Arterial Cocultures.Annals of biomedical engineering · 2016Article
- Regulation of vascular smooth muscle cell phenotype in three-dimensional coculture system by Jagged1-selective Notch3 signaling.Tissue engineering. Part A · 2014Article
- NOX2 in lung inflammation: quantum dot based in situ imaging of NOX2-mediated expression of vascular cell adhesion molecule-1.American journal of physiology. Lung cellular and molecular physiology · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The development of a functional, adherent endothelium is one of the major factors limiting the successful development of tissue engineered vascular grafts (TEVGs). The adhesion and function of endothelial cells (ECs) on smooth muscle cells (SMCs) are poorly understood. The goal of this research was to optimize conditions for the direct culture of endothelium on SMCs, and to develop an initial assessment of co-culture on EC function. The co-culture consisted of a culture substrate, a basal adhesion protein, a layer of porcine SMCs, a medial adhesion protein, and a layer of porcine ECs. Conditions that led to successful co-culture were: a polystyrene culture substrate, a quiescent state for SMCs, subconfluent density for SMC seeding and confluent density for EC seeding, and fibronectin (FN) for the basal adhesion protein. EC adhesion was not enhanced by addition of FN, collagen I, collagen IV or laminin (LN) to the medial layer. 3-D image reconstruction by confocal microscopy indicated that SMCs did not migrate over ECs and the cells were present in two distinct layers. Co-cultures could be consistently maintained for as long as 10 days. After exposure to 5 dyne/cm(2) for 7.5 h, ECs remained adherent to SMCs. PECAM staining indicated junction formation between ECs, but at a lower level than that observed with EC monocultures. Co-culturing ECs with SMCs did not change the growth rate of ECs, but EC DiI-Ac-LDL uptake was increased. Thus, a confluent and adherent layer of endothelium can be directly cultured on quiescent SMCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.