ArticleNeoplasia (New York, N.Y.)
Stability and heterogeneity of expression profiles in lung cancer specimens harvested following surgical resection.
Article in Neoplasia (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Phase II study of dasatinib in patients with advanced non-small-cell lung cancer.Journal of clinical oncology : official journal of the American Society of Clinical Oncology · 2010Trial
- Prospective validation of ORACLE, a clonal expression biomarker associated with survival of patients with lung adenocarcinoma.Nature cancer · 2025Article
- Tumor specimen cold ischemia time impacts molecular cancer drug target discovery.Cell death & disease · 2024Article
- The clonal expression genes associated with poor prognosis of liver cancer.Frontiers in genetics · 2022Article
- A clonal expression biomarker associates with lung cancer mortality.Nature medicine · 2019Article
- Deleterious effects of formalin-fixation and delays to fixation on RNA and miRNA-Seq profiles.Scientific reports · 2019Article
- Effects of Cold Ischemia on Gene Expression: A Review and Commentary.Biopreservation and biobanking · 2016Review
- Biobanking in genomic medicine.Archives of pathology & laboratory medicine · 2015Review
- World Endometriosis Research Foundation Endometriosis Phenome and Biobanking Harmonisation Project: IV. Tissue collection, processing, and storage in endometriosis research.Fertility and sterility · 2014Review
- Gene profiling and bioinformatics analyses reveal time course differential gene expression in surgically resected colorectal tissues.Oncology reports · 2014Article
- Article
- Tumor heterogeneity and resistance to EGFR-targeted therapy in advanced nonsmall cell lung cancer: challenges and perspectives.OncoTargets and therapy · 2014Review
- Public-private relationships in biobanking: a still underestimated key component of open innovation.Virchows Archiv : an international journal of pathology · 2014Review
- Impact of delay to cryopreservation on RNA integrity and genome-wide expression profiles in resected tumor samples.PloS one · 2013Article
- Effects of tissue handling on RNA integrity and microarray measurements from resected breast cancers.Journal of the National Cancer Institute · 2011Article
- Integrative computational biology for cancer research.Human genetics · 2011Review
- Biobanking human endometrial tissue and blood specimens: standard operating procedure and importance to reproductive biology research and diagnostic development.Fertility and sterility · 2011Article
- The role of gene expression profiling in early-stage non-small cell lung cancer.Journal of thoracic disease · 2010Article
- Article
- Developing a tissue resource to characterize the genome of pancreatic cancer.World journal of surgery · 2009Article
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9 authors.
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Abstract
One of the major concerns in microarray profiling studies of clinical samples is the effect of tissue sampling and RNA extraction on data. We analyzed gene expression in lung cancer specimens that were serially harvested from tumor mass and snap-frozen at several intervals up to 120 minutes after surgical resection. Global gene expression was profiled on cDNA microarrays, and selected stress and hypoxia-activated genes were evaluated using real-time reverse transcription polymerase chain reaction (RT-PCR). Remarkably, similar gene expression profiles were obtained for the majority of samples regardless of the time that had elapsed between resection and freezing. Real-time RT-PCR studies showed significant heterogeneity in the expression levels of stress and hypoxia-activated genes in samples obtained from different areas of a tumor specimen at one time point after resection. The variations between multiple samplings were significantly greater than those of elapsed time between sampling/freezing. Overall samples snap-frozen within 30 to 60 minutes of surgical resection are acceptable for gene expression studies, thus making sampling and snap-freezing of tumor samples in a routine surgical pathology laboratory setting feasible. However, sampling and pooling from multiple sites of each tumor may be necessary for expression profiling studies to overcome the molecular heterogeneity present in tumor specimens.
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