ArticleBMC molecular biology2005
Selection of reference genes for gene expression studies in human neutrophils by real-time PCR.
Article in BMC molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 114 papers.
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114 citing papers in PubMed, 241 citations in OpenAlex.
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- Platelet TLR7 is essential for the formation of platelet-neutrophil complexes and low-density neutrophils in lupus nephritis.Rheumatology (Oxford, England) · 2024Article
- Unveiling signaling pathways inducing MHC class II expression in neutrophils.Frontiers in immunology · 2024Article
- A Strategy for the Selection of RT-qPCR Reference Genes Based on Publicly Available Transcriptomic Datasets.Biomedicines · 2023Article
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- Selection and validation of reference genes for the normalization of quantitative real-time PCR in different muscle tissues of rabbits.BMC zoology · 2022Article
- IL-7/IL7R axis dysfunction in adults with severe community-acquired pneumonia (CAP): a cross-sectional study.Scientific reports · 2022Article
- Identification of appropriate housekeeping genes for gene expression studies in human renal cell carcinoma under hypoxic conditions.Molecular biology reports · 2022Article
- Evidence-based selection of reference genes for RT-qPCR assays in periodontal research.Clinical and experimental dental research · 2022Article
- Detection and Quantification of SARS-CoV-2 by Real-Time RT-PCR Assay.Methods in molecular biology (Clifton, N.J.) · 2022Article
54 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundReference genes, which are often referred to housekeeping genes, are frequently used to normalize mRNA levels between different samples. However the expression level of these genes may vary among tissues or cells, and may change under certain circumstances. Thus the selection of reference gene(s) is critical for gene expression studies. For this purpose, 10 commonly used housekeeping genes were investigated in isolated human neutrophils.
resultsInitial screening of the expression pattern demonstrated that 3 of the 10 genes were expressed at very low levels in neutrophils and were excluded from further analysis. The range of expression stability of the other 7 genes was (from most stable to least stable): GNB2L1 (Guanine nucleotide binding protein, beta polypeptide 2-like 1), HPRT1 (Hypoxanthine phosphoribosyl transferase 1), RPL32 (ribosomal protein L32), ACTB (beta-actin), B2M (beta-2-microglobulin), GAPD (glyceraldehyde-3-phosphate dehydrogenase) and TBP (TATA-binding protein). Relative expression levels of the genes (from high to low) were: B2M, ACTB, GAPD, RPL32, GNB2L1, TBP, and HPRT1.
conclusionOur data suggest that GNB2L1, HPRT1, RPL32, ACTB, and B2M may be suitable reference genes in gene expression studies of neutrophils.
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