ArticleThe Biochemical journal2005
Characterizing monoclonal antibody epitopes by filtered gene fragment phage display.
Article in The Biochemical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Comprehensive mapping of SARS-CoV-2 peptide epitopes for development of a highly sensitive serological test for total and neutralizing antibodies.Protein engineering, design & selection : PEDS · 2022Article
- Defining theFrontiers in microbiology · 2020Article
- An efficient ORF selection system for DNA fragment libraries based on split beta-lactamase complementation.PloS one · 2020Article
- Anti-type 2 transglutaminase antibodies as modulators of type 2 transglutaminase functions: a possible pathological role in celiac disease.Cellular and molecular life sciences : CMLS · 2018Review
- Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing.Journal of visualized experiments : JoVE · 2018Article
- Deep sequencing in library selection projects: what insight does it bring?Current opinion in structural biology · 2015Review
- Production of the Main Celiac Disease Autoantigen by Transient Expression in Nicotiana benthamiana.Frontiers in plant science · 2015Article
- Review
- Transglutaminase 2 expression is enhanced synergistically by interferon-γ and tumour necrosis factor-α in human small intestine.Clinical and experimental immunology · 2012Article
- A single conformational transglutaminase 2 epitope contributed by three domains is critical for celiac antibody binding and effects.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Filtering "genic" open reading frames from genomic DNA samples for advanced annotation.BMC genomics · 2011Article
- Rapid interactome profiling by massive sequencing.Nucleic acids research · 2010Article
- Mucosal tissue transglutaminase expression in celiac disease.Journal of cellular and molecular medicine · 2009Article
- Construction of miniantibodies for the in vivo study of human autoimmune diseases in animal models.BMC biotechnology · 2007Article
- Reduction of tissue transglutaminase autoantibody levels by gluten-free diet is associated with changes in subsets of peripheral blood lymphocytes in children with newly diagnosed coeliac disease.Clinical and experimental immunology · 2006Article
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present paper, we describe a novel approach to map monoclonal antibody epitopes, using three new monoclonal antibodies that recognize h-TG2 (human transglutaminase 2) as an example. The target gene was fragmented and cloned upstream of an antibiotic-resistance gene, in the vector pPAO2, to select for in-frame polypeptides. After removal of the antibiotic-resistance gene by Cre/Lox recombination, an antigen fragment phage display library was created and selected against specific monoclonal antibodies. Using the h-TG2 fragment library, we were able to identify epitopes. This technique can also be broadly applied to the study of protein-protein interactions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.