ArticleGene therapy2005
Library-based selection of retroviruses selectively spreading through matrix metalloprotease-positive cells.
Article in Gene therapy, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 27 citations in OpenAlex.
- Targeted and epigenetic therapies for acute myeloid leukemia treatment.Discover oncology · 2025Review
- Role of Tetra Amino Acid Motif Properties on the Function of Protease-Activatable Viral Vectors.ACS biomaterials science & engineering · 2016Article
- Antifibrotic properties of transarterial oncolytic VSV therapy for hepatocellular carcinoma in rats with thioacetamide-induced liver fibrosis.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Restriction of porcine endogenous retrovirus by porcine APOBEC3 cytidine deaminases.Journal of virology · 2011Article
- Cell entry targeting restricts biodistribution of replication-competent retroviruses to tumour tissue.Gene therapy · 2008Article
- Targeted cell entry of lentiviral vectors.Molecular therapy : the journal of the American Society of Gene Therapy · 2008Article
- Augmented transgene expression in transformed cells using a parvoviral hybrid vector.Cancer gene therapy · 2008Article
- Direct selection of targeted adenovirus vectors by random peptide display on the fiber knob.Gene therapy · 2007Article
- Identification of a retroviral receptor used by an envelope protein derived by peptide library screening.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Chronic gene delivery of interferon-inducible protein 10 through replication-competent retrovirus vectors suppresses tumor growth.Cancer gene therapy · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viruses conditionally replicating in cancer cells form an attractive novel class of antitumoral agents. To engineer such viruses infectivity can be coupled with proteolytic activity of the target cell by modifying the envelope (Env) protein of murine leukaemia virus (MLV) with blocking domains that prevent cell entry unless they are cleaved off by tumour-associated proteases like the matrix metalloproteases (MMP). Here we show that MLV variants selectively spreading through MMP-positive cells can be evolved from virus libraries, in which a standard MMP-2 substrate peptide connecting the blocking domain CD40L with the Env protein was diversified. Passaging the virus library on human fibrosarcoma or glioma cell lines resulted in the selection of about 10 virus clones, of which the three most frequent ones were shown to become activated by MMPs and to be replication competent on MMP-positive cells only. On these cells, the selected linker peptides improved the spreading by several orders of magnitude in vitro, as well as in tumour xenografts in vivo, approaching the kinetic of the unmodified wild-type virus. The data suggest that retroviral protease substrate libraries form a potent tool for the engineering of viruses conditionally replicating in a given cancer cell type of interest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.