ArticleGenetics2005
Optimizing the nucleotide sequence of a meiotic recombination hotspot in Schizosaccharomyces pombe.
Article in Genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 50 citations in OpenAlex.
- Mug20-Rec25-Rec27 binds DNA and enhances meiotic DNA break formation via phase-separated condensates.Nucleic acids research · 2025Article
- Measuring Meiotic Recombination Frequency in Schizosaccharomyces pombe Using an Engineered Genetic Interval.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Metabolic stress-induced long ncRNA transcription governs the formation of meiotic DNA breaks in the fission yeast fbp1 gene.PloS one · 2024Article
- Article
- Origin of translational control by eIF2α phosphorylation: insights from genome-wide translational profiling studies in fission yeast.Current genetics · 2021Review
- Gcn2 eIF2α kinase mediates combinatorial translational regulation through nucleotide motifs and uORFs in target mRNAs.Nucleic acids research · 2020Article
- Intragenic meiotic recombination in Schizosaccharomyces pombe is sensitive to environmental temperature changes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Article
- DNA sequence differences are determinants of meiotic recombination outcome.Scientific reports · 2019Article
- CDK contribution to DSB formation and recombination in fission yeast meiosis.PLoS genetics · 2019Article
- Physical basis for long-distance communication along meiotic chromosomes.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Pericentromere-Specific Cohesin Complex Prevents Meiotic Pericentric DNA Double-Strand Breaks and Lethal Crossovers.Molecular cell · 2018Article
- Sequence requirement of the ade6-4095 meiotic recombination hotspot in Schizosaccharomyces pombe.Genetica · 2018Article
- Correlation of Meiotic DSB Formation and Transcription Initiation Around Fission Yeast Recombination Hotspots.Genetics · 2017Article
- Unexpected DNA context-dependence identifies a new determinant of Chi recombination hotspots.Nucleic acids research · 2016Article
- Strong artificial selection in domestic mammals did not result in an increased recombination rate.Molecular biology and evolution · 2015Article
- Evolutionarily diverse determinants of meiotic DNA break and recombination landscapes across the genome.Genome research · 2014Article
- DNA intermediates of meiotic recombination in synchronous S. pombe at optimal temperature.Nucleic acids research · 2014Article
- The transcription factors Atf1 and Pcr1 are essential for transcriptional induction of the extracellular maltase Agl1 in fission yeast.PloS one · 2013Article
- The fission yeast FANCM ortholog directs non-crossover recombination during meiosis.Science (New York, N.Y.) · 2012Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The ade6-M26 mutation of Schizosaccharomyces pombe created a meiotic recombination hotspot. Previous analyses indicated that the heptamer 5'-ATGACGT-3' was necessary and sufficient for hotspot activity; the Atf1-Pcr1 transcription factor binds to this sequence and activates M26. After finding cases in which the M26 heptamer in ade6 was, surprisingly, not active as a hotspot, we used an in vitro selection method (SELEX) that revealed an 18-bp consensus sequence for Atf1-Pcr1 binding, 5'-GNVTATGACGTCATNBNC-3', containing the M26 heptamer at its core. Using this consensus sequence as a guide, we made mutations on each side of the heptamer at two separate sites in ade6. These mutations increased the intracellular hotspot activity of the heptamer, in some cases by >15-fold. These results show that M26, the eukaryotic recombination hotspot with the most precisely defined nucleotide sequence, is larger than previously thought, and they provide valuable information for clarifying the role of M26, and perhaps other hotspots, in meiotic recombination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.