ArticleJournal of bacteriology2005
Comparative study of class 1 integron and Vibrio cholerae superintegron integrase activities.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 97 citations in OpenAlex.
- High-throughput recovery of integron cassettes for gene discovery screens.Nature microbiology · 2026Article
- Trade-off between canonical and unusual recombination sites promotes diversity and stability of gene cassette arrays of mobile integrons.Scientific reports · 2026Article
- Bacterial natural transformation drives cassette shuffling and simplifies recombination in chromosomal integrons.Nucleic acids research · 2025Article
- Chromosomal integrons are genetically and functionally isolated units of genomes.Nucleic acids research · 2024Article
- Integron cassettes integrate into bacterial genomes via widespread non-classical attG sites.Nature microbiology · 2024Article
- A Class 4-like Chromosomal Integron Found inMicroorganisms · 2023Article
- Identification of integrons and gene cassette-associated recombination sites in bacteriophage genomes.Frontiers in microbiology · 2023Article
- IntegronFinder 2.0: Identification and Analysis of Integrons across Bacteria, with a Focus on Antibiotic Resistance in Klebsiella.Microorganisms · 2022Article
- Unbridled Integrons: A Matter of Host Factors.Cells · 2022Review
- The Natural History of Integrons.Microorganisms · 2021Review
- Predicting the taxonomic and environmental sources of integron gene cassettes using structural and sequence homology of attC sites.Communications biology · 2021Article
- Cassette recruitment in the chromosomal Integron of Vibrio cholerae.Nucleic acids research · 2021Article
- Article
- Structure-specific DNA recombination sites: Design, validation, and machine learning-based refinement.Science advances · 2020Article
- Structural heterogeneity of attC integron recombination sites revealed by optical tweezers.Nucleic acids research · 2019Article
- Dynamic stepwise opening of integron attC DNA hairpins by SSB prevents toxicity and ensures functionality.Nucleic acids research · 2017Article
- An att site-based recombination reporter system for genome engineering and synthetic DNA assembly.BMC biotechnology · 2017Article
- Diversity of gene cassettes and the abundance of the class 1 integron-integrase gene in sediment polluted by metals.Extremophiles : life under extreme conditions · 2016Article
- Unmasking the ancestral activity of integron integrases reveals a smooth evolutionary transition during functional innovation.Nature communications · 2016Article
- Using the class 1 integron-integrase gene as a proxy for anthropogenic pollution.The ISME journal · 2015Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Superintegrons (SIs) and multiresistant integrons (MRIs) have two main structural differences: (i) the SI platform is sedentary, while the MRI platform is commonly associated with mobile DNA elements and (ii) the recombination sites (attC) of SI gene cassette clusters are highly homogeneous, while those of MRI cassette arrays are highly variable in length and sequence. In order to determine if the latter difference was correlated with a dissimilarity in the recombination activities, we conducted a comparative study of the integron integrases of the class 1 MRI (IntI1) and the Vibrio cholerae SI (VchIntIA). We developed two assays that allowed us to independently measure the frequencies of cassette deletion and integration at the cognate attI sites. We demonstrated that the range of attC sites efficiently recombined by VchIntIA is narrower than the range of attC sites efficiently recombined by IntI1. Introduction of mutations into the V. cholerae repeats (VCRs), the attC sites of the V. cholerae SI cassettes, allowed us to map positions that affected the VchIntIA and IntI1 activities to different extents. Using a cointegration assay, we established that in E. coli, attI1-x-VCR recombination catalyzed by IntI1 was 2,600-fold more efficient than attIVch-x-VCR recombination catalyzed by VchIntIA. We performed the same experiments in V. cholerae and established that the attIVch-x-VCR recombination catalyzed by VchIntIA was 2,000-fold greater than the recombination measured in E. coli. Taken together, our results indicate that in the V. cholerae SI, the substrate recognition and recombination reactions mediated by VchIntIA might differ from the class 1 MRI paradigm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.