ArticleNucleic acids research2005
Use of mRNA- and protein-destabilizing elements to develop a highly responsive reporter system.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
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- The short conserved region-2 of LARP4 interacts with ribosome-associated RACK1 and promotes translation.bioRxiv : the preprint server for biology · 2024Article
- p65 signaling dynamics drive the developmental progression of hematopoietic stem and progenitor cells through cell cycle regulation.Nature communications · 2024Article
- Determining zebrafish dorsal organizer size by a negative feedback loop between canonical/non-canonical Wnts and Tlr4/NFκB.Nature communications · 2023Article
- Enhancement of Vivid-based photo-activatable Gal4 transcription factor in mammalian cells.Cell structure and function · 2023Article
- Spatio-temporal imaging of cell fate dynamics in single plant cells using luminescence microscope.Quantitative plant biology · 2022Article
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- Monitoring method for transgene expression in target tissue by blood sampling.Biotechnology reports (Amsterdam, Netherlands) · 2019Article
- A dual luciferase assay for evaluation of skin sensitizing potential of medical devices.Molecular biology reports · 2019Article
- In vivo study of gene expression with an enhanced dual-color fluorescent transcriptional timer.eLife · 2019Article
- Engineering protein-protein devices for multilayered regulation of mRNA translation using orthogonal proteases in mammalian cells.Nature communications · 2018Article
- HSF1 mediated stress response of heavy metals.PloS one · 2018Article
- An estradiol-inducible promoter enables fast, graduated control of gene expression in fission yeast.Yeast (Chichester, England) · 2017Article
- Photoactivatable CRISPR-Cas9 for optogenetic genome editing.Nature biotechnology · 2015Article
- Effects of receptor tyrosine kinase inhibitors on VEGF165 a- and VEGF165 b-stimulated gene transcription in HEK-293 cells expressing human VEGFR2.British journal of pharmacology · 2015Article
- Article
- Isl1 is a direct transcriptional target of Forkhead transcription factors in second-heart-field-derived mesoderm.Developmental biology · 2009Article
- Cloning-free regulated monitoring of reporter and gene expression.BMC molecular biology · 2009Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reporter assays are widely used in applications that require measurement of changes in gene expression over time (e.g. drug screening). With standard reporter vectors, the measurable effect of a treatment or compound (altered reporter activity) is substantially diluted and delayed, compared with its true effect (altered transcriptional activity). This problem is caused by the relatively long half-lives of both the reporter protein and its mRNA. As a result, the activities of compounds, ligands or treatments that have a relatively minor effect, or a substantial but transient effect, often remain undetected. To circumvent this problem, we introduced modular protein- and mRNA-destabilizing elements into a range of commonly used reporters. Our data show that both elements are required for maximal responses to both increases and decreases in transcriptional activity. The double-destabilized reporter vectors showed markedly improved performance in drug screening, kinetic assays and dose-response titrations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.