ArticleGenes & development2005
Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing.
Article in Genes & development, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 634 papers.
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Who cites it
634 citing papers in PubMed, 1,243 citations in OpenAlex.
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- Endogenous retrovirus IAP forms virus-like particles and traffics across the maternal-fetal barrier.bioRxiv : the preprint server for biology · 2025Article
- Human DICER1 hotspot mutation induces both loss and gain of miRNA function.Nature structural & molecular biology · 2025Article
- Human Dicer1 hotspot mutation induces both loss and gain of miRNA function.bioRxiv : the preprint server for biology · 2025Article
- Repression of AGO1 by AGO2 via let-7 microRNAs facilitates embryonic stem cell differentiation.RNA (New York, N.Y.) · 2025Article
- The biogenesis and regulation of animal microRNAs.Nature reviews. Molecular cell biology · 2025Review
- MicroRNA regulation of enteric nervous system development and disease.Trends in neurosciences · 2025Review
- The miR-290 and miR-302 clusters are essential for reprogramming of fibroblasts to induced pluripotent stem cells.Stem cells (Dayton, Ohio) · 2025Article
- The miR-290 and miR-302 clusters are essential for reprogramming of fibroblasts to induced pluripotent stem cells.bioRxiv : the preprint server for biology · 2024Article
- RNA-Mediated Regulation of Glycolysis in Embryonic Stem Cell Pluripotency and Differentiation.Current stem cell reports · 2024Article
- Determinants of minor satellite RNA function in chromosome segregation in mouse embryonic stem cells.The Journal of cell biology · 2024Article
- Combinatorial microRNA activity is essential for the transition of pluripotent cells from proliferation into dormancy.Genome research · 2024Article
- Evidence for Functional Roles of MicroRNAs in Lineage Specification During Mouse and Human Preimplantation Development.The Yale journal of biology and medicine · 2023Review
- The roles of miRNAs in adult skeletal muscle satellite cells.Free radical biology & medicine · 2023Review
- MicroRNAs Regulate CaCells · 2023Article
- Review
574 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dicer is the enzyme that cleaves double-stranded RNA (dsRNA) into 21-25-nt-long species responsible for sequence-specific RNA-induced gene silencing at the transcriptional, post-transcriptional, or translational level. We disrupted the dicer-1 (dcr-1) gene in mouse embryonic stem (ES) cells by conditional gene targeting and generated Dicer-null ES cells. These cells were viable, despite being completely defective in RNA interference (RNAi) and the generation of microRNAs (miRNAs). However, the mutant ES cells displayed severe defects in differentiation both in vitro and in vivo. Epigenetic silencing of centromeric repeat sequences and the expression of homologous small dsRNAs were markedly reduced. Re-expression of Dicer in the knockout cells rescued these phenotypes. Our data suggest that Dicer participates in multiple, fundamental biological processes in a mammalian organism, ranging from stem cell differentiation to the maintenance of centromeric heterochromatin structure and centromeric silencing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.