ArticleMolecular and cellular biology2005
A motif within SET-domain proteins binds single-stranded nucleic acids and transcribed and supercoiled DNAs and can interfere with assembly of nucleosomes.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
47 citing papers in PubMed, 75 citations in OpenAlex.
- Looping forward: exploring R-loop processing and therapeutic potential.FEBS letters · 2025Review
- SET DOMAIN GROUP 721 protein functions in saline-alkaline stress tolerance in the model rice variety Kitaake.Plant biotechnology journal · 2021Article
- The Trithorax group protein ASH1 requires a combination of BAH domain and AT hooks, but not the SET domain, for mitotic chromatin binding and survival.Chromosoma · 2021Article
- Epigenetic Attire in Ovarian Cancer: The Emperor's New Clothes.Cancer research · 2020Review
- PRC2 is high maintenance.Genes & development · 2019Review
- Theoretical analysis of Polycomb-Trithorax systems predicts that poised chromatin is bistable and not bivalent.Nature communications · 2019Article
- SIRT6-dependent cysteine monoubiquitination in the PRE-SET domain of Suv39h1 regulates the NF-κB pathway.Nature communications · 2018Article
- Transcription-associated events affecting genomic integrity.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Ctf4-related protein recruits LHP1-PRC2 to maintain H3K27me3 levels in dividing cells inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Article
- Nascent Connections: R-Loops and Chromatin Patterning.Trends in genetics : TIG · 2016Review
- Identification of SET Domain-Containing Proteins in Gossypium raimondii and Their Response to High Temperature Stress.Scientific reports · 2016Article
- Arabidopsis DNA polymerase ϵ recruits components of Polycomb repressor complex to mediate epigenetic gene silencing.Nucleic acids research · 2016Article
- Prevalent, Dynamic, and Conserved R-Loop Structures Associate with Specific Epigenomic Signatures in Mammals.Molecular cell · 2016Article
- R-loop: an emerging regulator of chromatin dynamics.Acta biochimica et biophysica Sinica · 2016Review
- Review
- Structural analysis of an active fungal PRC2.Nucleus (Austin, Tex.) · 2016Article
- Imprinting control regions (ICRs) are marked by mono-allelic bivalent chromatin when transcriptionally inactive.Nucleic acids research · 2016Article
- The SET Domain Is Essential for Metnase Functions in Replication Restart and the 5' End of SS-Overhang Cleavage.PloS one · 2015Article
- Repeat-mediated epigenetic dysregulation of the FMR1 gene in the fragile X-related disorders.Frontiers in genetics · 2015Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
Abstract
The evolutionary conserved SET domain is present in many eukaryotic chromatin-associated proteins, including some members of the trithorax (TrxG) group and the polycomb (PcG) group of epigenetic transcriptional regulators and modifiers of position effect variegation. All SET domains examined exhibited histone lysine methyltransferase activity, implicating these proteins in the generation of epigenetic marks. However, the mode of the initial recruitment of SET proteins to target genes and the way that their association with the genes is maintained after replication are not known. We found that SET-containing proteins of the SET1 and SET2 families contain motifs in the pre-SET region or at the pre-SET-SET and SET-post-SET boundaries which very tightly bind single-stranded DNA (ssDNA) and RNA. These motifs also bind stretches of ssDNA generated by superhelical tension or during the in vitro transcription of duplex DNA. Importantly, such binding withstands nucleosome assembly, interfering with the formation of regular nucleosomal arrays. Two representatives of the SUV39 SET family, SU(VAR)3-9 and G9a, did not bind ssDNA. The trxZ11 homeotic point mutation, which is located within TRX SET and disrupts embryonic development, impairs the ssDNA binding capacity of the protein. We suggest that the motifs described here may be directly involved in the biological function(s) of SET-containing proteins. The binding of single-stranded nucleic acids might play a role in the initial recruitment of the proteins to target genes, in the maintenance of their association after DNA replication, or in sustaining DNA stretches in a single-stranded configuration to allow for continuous transcription.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.