ArticleProceedings of the National Academy of Sciences of the United States of America2005
Reconstitution of papillomavirus E2-mediated plasmid maintenance in Saccharomyces cerevisiae by the Brd4 bromodomain protein.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 42 citations in OpenAlex.
- Structural basis for P-TEFb association with BRD4.bioRxiv : the preprint server for biology · 2026Article
- Interaction with TopBP1 Is Required for Human Papillomavirus 16 E2 Plasmid Segregation/Retention Function during Mitosis.Journal of virology · 2022Article
- Article
- Evidence supporting a role for TopBP1 and Brd4 in the initiation but not continuation of human papillomavirus 16 E1/E2-mediated DNA replication.Journal of virology · 2015Article
- Brd4-mediated nuclear retention of the papillomavirus E2 protein contributes to its stabilization in host cells.Viruses · 2014Article
- Phosphorylation of HPV-16 E2 at serine 243 enables binding to Brd4 and mitotic chromosomes.PloS one · 2014Article
- Current understanding of the role of the Brd4 protein in the papillomavirus lifecycle.Viruses · 2013Review
- Analysis of the papillomavirus E2 and bromodomain protein Brd4 interaction using bimolecular fluorescence complementation.PloS one · 2013Article
- Small molecule inhibitors of human papillomavirus protein - protein interactions.The open virology journal · 2011Article
- Abrogation of the Brd4-positive transcription elongation factor B complex by papillomavirus E2 protein contributes to viral oncogene repression.Journal of virology · 2010Article
- Mitotic chromosome interactions of Epstein-Barr nuclear antigen 1 (EBNA1) and human EBNA1-binding protein 2 (EBP2).Journal of cell science · 2009Article
- Regulation of aurora B expression by the bromodomain protein Brd4.Molecular and cellular biology · 2009Article
- Role of Kaposi's sarcoma-associated herpesvirus C-terminal LANA chromosome binding in episome persistence.Journal of virology · 2009Article
- The human papillomavirus type 8 E2 tethering protein targets the ribosomal DNA loci of host mitotic chromosomes.Journal of virology · 2009Article
- The EBNA1 protein of Epstein-Barr virus functionally interacts with Brd4.Journal of virology · 2008Article
- Varying efficiency of long-term replication of papillomaviruses in Saccharomyces cerevisiae.Virology · 2008Article
- Replication and partitioning of papillomavirus genomes.Advances in virus research · 2008Review
- Brd4-independent transcriptional repression function of the papillomavirus e2 proteins.Journal of virology · 2007Article
- Recent advances in the search for antiviral agents against human papillomaviruses.Antiviral therapy · 2007Review
- Brd4 is required for e2-mediated transcriptional activation but not genome partitioning of all papillomaviruses.Journal of virology · 2006Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
The papillomavirus E2 protein functions in viral transcriptional regulation, DNA replication, and episomal genome maintenance. Viral genomes are maintained in dividing cells by attachment to mitotic chromosomes by means of the E2 protein. To investigate the chromosomal tethering function of E2, plasmid stability assays were developed in Saccharomyces cerevisiae to determine whether the E2 protein could maintain plasmids containing the yeast autonomous replication sequence replication element but with the centromeric element replaced by E2-binding sites. E2 expression was not sufficient to maintain such plasmids, but plasmid stability could be rescued by expression of the mammalian protein Brd4. In the presence of both Brd4 and E2 proteins, plasmids with multiple E2-binding sites were stable without selection. S. cerevisiae encodes a homolog of Brd4 named Bdf1 that does not contain the C-terminal domain that interacts with the E2 protein. A fusion protein of Bdf1 and the Brd4 C-terminal "tail" could support E2-mediated plasmid maintenance in yeast. Using a panel of mutated E2 proteins, we determined that plasmid stability required the ability of E2 to bind DNA and to interact with Brd4 and mammalian mitotic chromosomes but did not require its replication initiation and transactivation functions. The S. cerevisiae-based plasmid maintenance assays described here are invaluable tools for dissecting mechanisms of episomal viral genome replication and screening for additional host protein factors involved in plasmid maintenance.
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