ArticleBMC infectious diseases2005
Detection of virulence genes in Malaysian Shigella species by multiplex PCR assay.
Article in BMC infectious diseases, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 86 citations in OpenAlex.
- Virulence factors associated with pediatric shigellosis in Brazilian Amazon.BioMed research international · 2014Trial
- Virulence factors and mechanisms of antimicrobial resistance in Shigella strains from periurban areas of Lima (Peru).International journal of medical microbiology : IJMMTrial
- Fecal sludge and adjacent drinking water as reservoirs of multidrug resistant ESBL-producing pathogenic Escherichia coli in Rohingya Camps, Bangladesh.Scientific reports · 2026Article
- Lactose fermenting enteroinvasive Escherichia coli from diarrhoeal cases confers enhanced virulence.Scientific reports · 2025Article
- Integrating Metagenomic and Culture-Based Techniques to Detect Foodborne Pathogens and Antimicrobial Resistance Genes in Malaysian Produce.Foods (Basel, Switzerland) · 2025Article
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- Etiology and risk factors for diarrheal disease amongst rural and peri-urban populations in Cambodia, 2012-2018.PloS one · 2023Article
- ESBL ProducingFrontiers in public health · 2021Article
- Differential gene expression profile ofFuture science OA · 2020Article
- Virulence-Related Gene Distribution AmongInfection and drug resistance · 2020Article
- Evaluation of LAMP for detection ofAccess microbiology · 2020Article
- Extended-Spectrum Beta-Lactamase-ProducingFrontiers in public health · 2020Article
- Virulence gene profiles ofPathogens and global health · 2019Article
- Extensive inter-strain diversity among clinical isolates ofGut pathogens · 2019Article
- Virulence Gene Profile and Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) of Enteroinvasive Escherichia coli (EIEC) Isolates From Patients With Diarrhea in Kerman, Iran.Jundishapur journal of microbiology · 2016Article
- Article
- Occurrence of genes encoding enterotoxins in uropathogenic Escherichia coli isolates.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2015Article
- Clinical and microbiological profiles of shigellosis in children.Journal of health, population, and nutrition · 2014Article
- Development of Multiplex PCR for Simultaneous Detection of Three Pathogenic Shigella Species.Iranian journal of public health · 2014Article
- Pentaplex PCR assay for detection of hemorrhagic bacteria from stool samples.Journal of clinical microbiology · 2014Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn Malaysia, Shigella spp. was reported to be the third commonest bacterial agent responsible for childhood diarrhoea. Currently, isolation of the bacterium and confirmation of the disease by microbiological and biochemical methods remain as the "gold standard". This study aimed to detect the prevalence of four Shigella virulence genes present concurrently, in randomly selected Malaysian strains via a rapid multiplex PCR (mPCR) assay.
methodsA mPCR assay was designed for the simultaneous detection of chromosomal- and plasmid-encoded virulence genes (set1A, set1B, ial and ipaH) in Shigella spp. One hundred and ten Malaysian strains (1997-2000) isolated from patients from various government hospitals were used. Reproducibility and sensitivity of the assay were also evaluated. Applicability of the mPCR in clinical settings was tested with spiked faeces following preincubation in brain heart infusion (BHI) broth.
resultsThe ipaH sequence was present in all the strains, while each of the set1A, set1B and ial gene was present in 40% of the strains tested. Reproducibility of the mPCR assay was 100% and none of the non-Shigella pathogens tested in this study were amplified. The mPCR could detect 100 colony-forming units (cfu) of shigellae per reaction mixture in spiked faeces following preincubation.
conclusionsThe mPCR system is reproducible, sensitive and is able to identify pathogenic strains of shigellae irrespective of the locality of the virulence genes. It can be easily performed with a high throughput to give a presumptive identification of the causal pathogen.
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