ArticleProceedings of the National Academy of Sciences of the United States of America2005
Interaction of BIG2, a brefeldin A-inhibited guanine nucleotide-exchange protein, with exocyst protein Exo70.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
- Endosidin 2 accelerates PIN2 endocytosis and disturbs intracellular trafficking of PIN2, PIN3, and PIN4 but not of SYT1.PloS one · 2020Article
- Oligomerization of the Sec7 domain Arf guanine nucleotide exchange factor GBF1 is dispensable for Golgi localization and function but regulates degradation.American journal of physiology. Cell physiology · 2016Article
- Regulating the large Sec7 ARF guanine nucleotide exchange factors: the when, where and how of activation.Cellular and molecular life sciences : CMLS · 2014Review
- Filamin A mediated Big2 dependent endocytosis: From apical abscission to periventricular heterotopia.Tissue barriers · 2014Article
- Brefeldin A-inhibited ADP-ribosylation factor activator BIG2 regulates cell migration via integrin β1 cycling and actin remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Article
- Effects of brefeldin A-inhibited guanine nucleotide-exchange (BIG) 1 and KANK1 proteins on cell polarity and directed migration during wound healing.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Poliovirus replication requires the N-terminus but not the catalytic Sec7 domain of ArfGEF GBF1.Cellular microbiology · 2010Article
- An aPKC-exocyst complex controls paxillin phosphorylation and migration through localised JNK1 activation.PLoS biology · 2009Article
- Large Arf1 guanine nucleotide exchange factors: evolution, domain structure, and roles in membrane trafficking and human disease.Molecular genetics and genomics : MGG · 2009Review
- The role of the exocyst in matrix metalloproteinase secretion and actin dynamics during tumor cell invadopodia formation.Molecular biology of the cell · 2009Article
- Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- A C-terminal sequence in the guanine nucleotide exchange factor Sec7 mediates Golgi association and interaction with the Rsp5 ubiquitin ligase.The Journal of biological chemistry · 2008Article
- Natural products as an inspiration in the diversity-oriented synthesis of bioactive compound libraries.Natural product reports · 2008Review
- Unfolded protein response and cell death after depletion of brefeldin A-inhibited guanine nucleotide-exchange protein GBF1.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Phosphatidylinositol 4,5-bisphosphate mediates the targeting of the exocyst to the plasma membrane for exocytosis in mammalian cells.Molecular biology of the cell · 2007Article
- BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein, is required for correct glycosylation and function of integrin beta1.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Association of brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2) with recycling endosomes during transferrin uptake.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Effect of protein kinase A on accumulation of brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) in HepG2 cell nuclei.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Filamin A and Big2: a shared endocytic pathway.BioarchitectureArticle
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Guanine nucleotide-exchange proteins activate ADP-ribosylation factors by accelerating the replacement of bound GDP with GTP. Mammalian brefeldin A-inhibited guanine nucleotide-exchange proteins, BIG1 and BIG2, are important activators of ADP-ribosylation factors for vesicular trafficking. To identify proteins that interact with BIG2, we used cDNA constructs encoding BIG2 sequences in a yeast two-hybrid screen of a human heart library. Clone p2-5-3, encoding a form of human exocyst protein Exo70, interacted with BIG2 amino acids 1-643 and 1-832, but not 644-832, which was confirmed by coimmunoprecipitation of in vitro-translated BIG2 N-terminal segments and 2-5-3. By immunofluorescence microscopy, endogenous BIG2 and Exo70 in HepG2 cells were visualized at Golgi membranes and apparently at the microtubule-organizing center (MTOC). Both were identified in purified centrosomes. Immunoreactive Exo70 and BIG2 partially or completely overlapped with gamma-tubulin at the MTOC in cells inspected by confocal microscopy. In cells incubated with brefeldin A, most of the BIG2, Exo70, and trans-Golgi protein p230 were widely dispersed from their perinuclear concentrations, but small amounts always remained, apparently at the MTOC. After disruption of microtubules with nocodazole, BIG2 and Exo70 were widely distributed in cells and remained only partially colocalized with p230, BIG2 more so than Exo70. We conclude that in HepG2 cells BIG2 and Exo70 interact in trans-Golgi network and centrosomes, as well as in exocyst structures or complexes that move along microtubules to the plasma membrane, consistent with a functional association in both early and late stages of vesicular trafficking.
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