ArticleRNA (New York, N.Y.)2005
RNA polymerase II synthesizes antisense RNA in Plasmodium falciparum.
Article in RNA (New York, N.Y.), 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 65 citations in OpenAlex.
- New insights on Plasmodium gene expression from direct RNA sequencing.Trends in parasitology · 2026Review
- Differential expression of mitochondria-associated genes in clinical samples of Plasmodium falciparum showing severe manifestations.BMC genomics · 2026Article
- Integrative transcriptomic and machine learning approaches to decipher mitochondrial gene regulation in severe Plasmodium vivax malaria.Malaria journal · 2025Article
- Emerging biology of noncoding RNAs in malaria parasites.PLoS pathogens · 2022Review
- Non-coding RNAs in malaria infection.Wiley interdisciplinary reviews. RNA · 2022Review
- The RNA structurome in the asexual blood stages of malaria pathogenRNA biology · 2021Article
- Noncoding RNAs in Apicomplexan Parasites: An Update.Trends in parasitology · 2020Review
- Re-annotation of the Theileria parva genome refines 53% of the proteome and uncovers essential components of N-glycosylation, a conserved pathway in many organisms.BMC genomics · 2020Article
- Long Non-Coding RNAs in the Regulation of Gene Expression: Physiology and Disease.Non-coding RNA · 2019Review
- Plasmodium falciparum specific helicase 2 is a dual, bipolar helicase and is crucial for parasite growth.Scientific reports · 2019Article
- tRNA epitranscriptomics and biased codon are linked to proteome expression inMolecular systems biology · 2018Article
- Transcripts in the Plasmodium Apicoplast Undergo Cleavage at tRNAs and Editing, and Include Antisense Sequences.Protist · 2016Article
- Landscape and Dynamics of Transcription Initiation in the Malaria Parasite Plasmodium falciparum.Cell reports · 2016Article
- Article
- Genome-wide RIP-Chip analysis of translational repressor-bound mRNAs in the Plasmodium gametocyte.Genome biology · 2014Article
- Strand-specific RNA-Seq reveals widespread and developmentally regulated transcription of natural antisense transcripts in Plasmodium falciparum.BMC genomics · 2014Article
- RNA-Seq analysis of splicing in Plasmodium falciparum uncovers new splice junctions, alternative splicing and splicing of antisense transcripts.Nucleic acids research · 2011Article
- H2A.Z demarcates intergenic regions of the plasmodium falciparum epigenome that are dynamically marked by H3K9ac and H3K4me3.PLoS pathogens · 2010Article
- PfSRPK1, a novel splicing-related kinase from Plasmodium falciparum.The Journal of biological chemistry · 2010Article
- PfeIF4E and PfeIF4A colocalize and their double-stranded RNA inhibits Plasmodium falciparum proliferation.Communicative & integrative biology · 2010Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The recent identification of antisense RNA in the transcriptomes of many eukaryotes has generated enormous interest. The presence of antisense RNA in Plasmodium falciparum, the causative agent of severe malaria, remains controversial. Elucidation of the mechanism of antisense RNA in P. falciparum synthesis is critical in order to demonstrate the origin and function of these transcripts. Therefore, a systematic analysis of antisense and sense RNA synthesis was performed using direct labeling experiments. Nuclear run on experiments with single-stranded DNA probes demonstrated that antisense RNA is synthesized in the nucleus at several genomic loci. Antisense RNA synthesis is sensitive to the potent RNA polymerase II inhibitor alpha-amanitin. Antisense and sense transcription was also detected in nuclei isolated from synchronized parasites, suggesting concurrent synthesis. In summary, our experiments directly demonstrate that antisense RNA synthesis is a common transcriptional phenomenon in P. falciparum, and is catalyzed by RNA polymerase II.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.